{"created":"2023-07-27T06:55:02.911085+00:00","id":50687,"links":{},"metadata":{"_buckets":{"deposit":"7a4959f1-875e-4026-b6df-4237548ef404"},"_deposit":{"created_by":18,"id":"50687","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50687"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050687","sets":["2812:2813:2826"]},"author_link":["20229","83237"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-05","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"12p.","bibliographicVolumeNumber":"2006-2007","bibliographic_titles":[{"bibliographic_title":"平成19(2007)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2007 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒトパピローマウイルス(HPV)E4蛋白は、ウイルスの粒子の放出に重要な蛋白で上皮細胞内で大量に発現する。本研究では、HPV感染初期病変におけるE4蛋白の発現と免疫応答の関係を調査し、HPVに対する免疫の回避機構を明らかにすることが目的である。研究に先立ち、2006年度には、CIN患者の感染HPVタイプを明らかにするため、GP5+/6+PCR法に改良を加えて方法でHPV-DNA診断とsequence法によるタイプ判定を行った。その後、新しく市販されている3種類のHPV typing kitを用いた結果を比較検討したところ、検査法によりHPVタイプ結果が異なる場合があること、異なるHPVタイプの混合感染が予想以上に多いことを発見した。特に混合型感染例におけるHPVタイプ一致率が低い傾向がみられる。現在、これらの調査結果を解析し、各kit間の違いを明らかにしている段階である。\nHPV11,16,18,31,33,45,56型に対する抗E4抗体を英国の共同研究者から確保した。HPVタイプの判明している子宮頚癌の前癌病変である頸部上皮内新生物(CIN)におけるE4蛋白発現の検討を行ったところ、これまでの報告と同様にCINの中層から表層部にかけてE4蛋白の局在が観察された。調べて範囲内ではE4蛋白の発現は多いが、間質にある免疫担当細胞などにも染色されるため、これが単なる交差反応か意義のある所見かどうかについて検討している。今後、HPV型の判定結果を確定させてから、E4抗体のHPV型特異性についての検討を行う予定である。E4蛋白の免疫染色はHPV感染の診断に応用できる可能性があり、新しいHPVワクチンのターゲットとしてE4蛋白が有効である可能性がある。","subitem_description_type":"Abstract"},{"subitem_description":"Certain human papillomavirus types named high-risk types are thought to cause cervical cancer and cervical intraepithelial neoplasia (CIN). It has been recently known that E4 proteins of these types are abundantly expressed in CIN epithelium. However, such expression has not been investigated extensively in clinical samples. This study is to examine the expression pattern using E4 antibodies for specific HPV types.\nWe obtained seven different polyclonal antibodies against E4 proteins of HPV types 11, 16, 18, 31, 33, 56, which were produced from immunized rabbits and purified. lb start this experiment, we tried to do HPV genotyping in cytological samples using three different HPV genotyping methods in women who were abnormal in cytology. These methods are now commercially available in Japan. We found that different assays have shown discordant results in some of the samples examined. Discordant results were more frequently observed in the cases infected with different HPV types. This type of multiple HPV type infection were more common in women having abnormal cytology as we have previously expected. Now we are going to analyze more detail in these eases showing discordant results. This is a crucial step for further experiment for E4 study.\nE4 expression has been confirmed on epithelium from middle to superficial layers in most of CIN1 and CIN2, 3 lesions in which HPV types were determined. Abundant expression was seen in some cases. However, some immune cells were also stained by these antibodies. We have to figure out the positive reaction to immune cell is merely due to cross-reaction or due to presence of E4 in these immune cells. Furthermore, we have to test type-specificity of these antibodies in immune-staining. E4 protein immunostaining method may be useful for diagnostic purpose of high-risk HPV infection, and furthermore E4 might be a good target for therapeutic HPV vaccine.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18591827, 研究期間(年度):2006-2007","subitem_description_type":"Other"},{"subitem_description":"出典：「子宮頚部における高リスク型ヒトパピローマウイルスE4蛋白の発現と免疫応答」研究成果報告書　課題番号18591827\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056995","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医学系研究科"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=30272975"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=30272975","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591827/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591827/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18591827/185918272007kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18591827/185918272007kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-05"}],"displaytype":"detail","filename":"ME-PR-SASAGAWA-T-kaken 2008-12p.pdf","filesize":[{"value":"870.7 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SASAGAWA-T-kaken 2008-12p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50687/files/ME-PR-SASAGAWA-T-kaken 2008-12p.pdf"},"version_id":"830ec5ae-ea18-4bbf-a60d-0cf4aea53155"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"子宮頚部における高リスク型ヒトパピローマウイルスE4蛋白の発現と免疫応答","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"子宮頚部における高リスク型ヒトパピローマウイルスE4蛋白の発現と免疫応答"},{"subitem_title":"Expression and immunoreactivity of high-risk human papillomavirus E4 prtein","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2826"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-05"},"publish_date":"2020-03-05","publish_status":"0","recid":"50687","relation_version_is_last":true,"title":["子宮頚部における高リスク型ヒトパピローマウイルスE4蛋白の発現と免疫応答"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:23:39.567594+00:00"}