{"created":"2023-07-27T06:55:16.698908+00:00","id":51056,"links":{},"metadata":{"_buckets":{"deposit":"e15d8ddf-59e1-40d8-81b2-086b7ba58f53"},"_deposit":{"created_by":18,"id":"51056","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"51056"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00051056","sets":["2812:2813:2837"]},"author_link":["21834","92452"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-08","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"3p.","bibliographicVolumeNumber":"1995-1996","bibliographic_titles":[{"bibliographic_title":"平成8(1996)年度 科学研究費補助金 基盤研究(B) 研究成果報告書概要"},{"bibliographic_title":"1996 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Anti-gene is a potent inhibitor of transcriptional promoter activity and subsequent gene expression. Since the number of copies of DNA is much fewer than those of transcribed mRNA,anti-gene oligonucleotides targeted against the promoter region would have an advantage over antisense oligonucleotides with an mRNA as a target. This property has been exploited to suppress the expression of a variety of oncogenes for regulating tumor proliferation or viral activities. We developed a novel retroviral vector designed to express human c-erb Banti-gene RNA and to reduce the promoter activity in the cells. Mouse fibroblast NIH3T3 cells were stably transfected with an expression construct containing a truncated human c-erb B gene promoter fused to the firefly luciferase reporter gene. Addition to these cells of the c-erb B anti-gene retroviral vector targeted to the 26 bp pyrimidine-rich element in the human c-erb B gene promoter resulted in a dose-dependent decrease in the luciferase activity of the cells. The results suggest that the reduction of the luciferase activity is due to the effect of the transcribed human c-erb B anti-gene RNA on initiation of transcription of the luciferase gene. The anti-gene RNA produced by a retroviral vector is expected to suppress the expression of an appropriate oncogene and to retard the growth of tumor cells in vivo.","subitem_description_type":"Abstract"},{"subitem_description":"従来よりわれわれは、c-erbB遺伝子のプロモーター領域に作用するアンチジーン合成DNAを作成し分子間三重鎖DNAの形成を試み、転写抑制によるc-erbB遺伝子発現抑制効果を検討してきた。c-erbB遺伝子のプロモーター領域のSP-1結合部位とCAP部位を含む26bpの領域を標的とした場合に低濃度の合成DNAで遺伝子の発現抑制が示され、これらの効果を報告してきた。この方法においては代謝、毒性に関して顕著な問題はないとされているが、腫瘍性疾患に対してはどのようにして効率良く発現させるかについてさらに工夫が必要であった。\nウイルスベクターにより発現されるアンチジーンRNAの転写阻害効果については従来報告がなく、その効果を検討するため、組み換えレトロウイルスベクターを構築しin vitroでの転写阻害について検討した。CMVプロモーターによりc-erbBアンチジーンを発現するウイルスベクターゲノムpLNCAを構築し、パッケージング細胞psi-CREを用いてアンチジーンレトロウイルスベクターLNCAを得た。一方、EGFRプロモーターにてルシフェラーゼ遺伝子を発現するベクターゲノムpLNELをマウス繊維芽細胞NIH3T3に導入し、この遺伝子組換え体細胞にウイルスベクターLNCAの反復感染を行い経時的にルシフェラーゼ酵素活性の測定を行った。LNCA感染後48時間で量依存性に酵素活性が減少し、MOI=10の条件下にて約50%のルシフェラーゼ発現阻害効果が認められた。レトロウイルスベクターにより発現されるアンチジーンRNAにより、オリゴヌクレオチドと同様に転写阻害効果が得られることが示唆された。転写阻害に必要な力価やウイルスの投与時期などの条件についてさらに検討中である。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:07457310, 研究期間(年度):1995-1996","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「アンチジーン発現ベクターを用いた悪性グリオーマに対する遺伝子療法」課題番号07457310\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-07457310/074573101996kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00057359","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90026948"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90026948","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457310/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457310/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-07457310/074573101996kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-07457310/074573101996kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-20"}],"displaytype":"detail","filename":"ME-PR-YAMASHITA-J-kaken 1999-3p.pdf","filesize":[{"value":"118.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-YAMASHITA-J-kaken 1999-3p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/51056/files/ME-PR-YAMASHITA-J-kaken 1999-3p.pdf"},"version_id":"da075bf6-7571-4e8d-adcb-f49fb71cac9e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"アンチジーン発現ベクターを用いた悪性グリオーマに対する遺伝子療法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アンチジーン発現ベクターを用いた悪性グリオーマに対する遺伝子療法"},{"subitem_title":"Inhibition of Gene Expression from the Human c-erb B Gene Promoter by a Retroviral Vector Expressing Anti-gene RNA","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2837"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-20"},"publish_date":"2022-05-20","publish_status":"0","recid":"51056","relation_version_is_last":true,"title":["アンチジーン発現ベクターを用いた悪性グリオーマに対する遺伝子療法"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:04:19.554480+00:00"}