{"created":"2023-07-27T06:55:20.498857+00:00","id":51144,"links":{},"metadata":{"_buckets":{"deposit":"6d005f37-cb90-4c78-b893-a7911055b873"},"_deposit":{"created_by":18,"id":"51144","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"51144"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00051144","sets":["2812:2813:4085"]},"author_link":["2653","77197"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"7p.","bibliographicVolumeNumber":"2019-04-01 - 2022-03-31","bibliographic_titles":[{"bibliographic_title":"令和3(2021)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"},{"bibliographic_title":"2021 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakao, Shinji"}],"nameIdentifiers":[{"nameIdentifier":"77197","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70217660","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70217660"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To identify an autoantigen presented by HLA-B4002 on hematopoietic stem progenitor cells (HSPCs) of patients with acquired aplastic anemia (AA), we established T-cell receptor (TCR) transfectants using T cells from healthy individuals and TCR cDNA derived from T cells that predominantly proliferated in the bone marrow of an AA patient carrying HLA-B*40:02, and examined specific response to peptides bound to HLA-B4002 on a leukemia cell line K562 that were transfected with HLA-B*40:02. Two kinds of TCR transfectants specifically recognized one of the HLA-B4002-bound peptides that are derived from a cytoplasmic protein (protein X), which is abundantly expressed by HSPCs. We prepared a peptide-HLA-B4002 tetramer and are now screening peripheral blood T cells of AA patients with HLA-B*40:02 for the presence of the peptide-specific CD8+ T cells.\nIn a separate experiment, we found that HSPCs of AA patients possessing HLA-DR15 frequently lacked HLA-DR through epigenetic mechanisms.","subitem_description_type":"Abstract"},{"subitem_description":"再生不良性貧血（AA）の自己抗原提示にもっとも深く関わっているHLA-B*40:02陽性患者の骨髄中に同定したT細胞レセプターのcDNAを健常者のTリンパ球に導入することにより、AA患者の造血幹前駆細胞（HSPC）をHLA-B4002拘束性に傷害するT細胞トランスフェクタントを作製した。これを用いて、HLA-B4002を発現させた白血病細胞株（K562）のHLA-B4002結合ペプチドをスクリーニングしたところ、HSPCが高発現している蛋白Xに選択的に反応することが分かった。また、HLA-DR15陽性AA患者のHSPCでは、HLA-DR発現がエピジェネティックに低下していることを見出した。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:19H03686, 研究期間(年度):2019-04-01 - 2022-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「再生不良性貧血におけるHLAクラスIアレル拘束性自己抗原の同定」課題番号19H03686\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19H03686/19H03686seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00057447","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=70217660"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=70217660","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03686/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03686/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19H03686/19H03686seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19H03686/19H03686seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中尾, 眞二"}],"nameIdentifiers":[{"nameIdentifier":"2653","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70217660","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70217660"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-02"}],"displaytype":"detail","filename":"ME-PR-NAKAO-S-kaken 2022-7p.pdf","filesize":[{"value":"97.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-NAKAO-S-kaken 2022-7p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/51144/files/ME-PR-NAKAO-S-kaken 2022-7p.pdf"},"version_id":"0365a767-9a9f-4593-909f-f30fd5d75149"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"再生不良性貧血におけるHLAクラスIアレル拘束性自己抗原の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"再生不良性貧血におけるHLAクラスIアレル拘束性自己抗原の同定"},{"subitem_title":"Identification of autoantigens presented by specific HLA class I alleles in aplastic anemia","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4085"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-02"},"publish_date":"2023-02-02","publish_status":"0","recid":"51144","relation_version_is_last":true,"title":["再生不良性貧血におけるHLAクラスIアレル拘束性自己抗原の同定"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:15:49.758073+00:00"}