{"created":"2023-07-27T06:55:23.993884+00:00","id":51231,"links":{},"metadata":{"_buckets":{"deposit":"c2a67e7a-a9df-43b8-b34d-3b96635baa59"},"_deposit":{"created_by":18,"id":"51231","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"51231"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00051231","sets":["2812:2813:4014"]},"author_link":["88146","88144"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"7p.","bibliographicVolumeNumber":"2018-04-01 - 2021-03-31","bibliographic_titles":[{"bibliographic_title":"令和2(2020)年度 科学研究費補助金 若手研究 研究成果報告書"},{"bibliographic_title":"2020 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"肝細胞増殖因子（HGF）は、がん細胞膜上の受容体METに結合し、シグナルを活性化することで、複数の悪性形質を誘導することが知られている。本課題は、活性型HGFに選択的に結合し、活性を阻害する特殊環状ペプチド (HiP8)を用いて、HGFによって形成されるがん微小環境を描出し、腫瘍特性を反映した診断法を確立することを目的に行われた。検討の結果、ビオチンを標識することで、腫瘍組織内の活性型HGFを選択的に検出する病理診断プローブとして使用できること、また金属キレータを修飾し、放射性同位元素であるCu-64を配位することで、低侵襲な診断法であるPETの分子プローブとして利用できることを明らかにした。","subitem_description_type":"Abstract"},{"subitem_description":"Aberrant activation of HGF and its receptor MET signaling is well-known to participate in the acquired resistance to molecular targeted drugs. However, current diagnostic approaches to know activation of HGF-MET pathway are insufficient. We recently identified a macrocyclic peptide, HGF-inhibitory peptide-8 (HiP8) which selectively binds to biologically active HGF. In this study, we attempted to specifically detect active HGF in tumor microenvironments for diagnosis by using HiP8. In immunohistochemical staining of active HGF in human cancer tissues using biotinylated HiP8, the HiP8-proved active HGF showed good correlation with staining scores and localization for MET activation, compared to staining of total HGF. Moreover, in tumor-xenograft models using human HGF-knock-in mice, Cu-64-labeled HiP-8 accumulated in xenografts overexpressing HGF at higher levels than those in HGF-negative xenografts.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18K15306, 研究期間(年度):2018-04-01 - 2021-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「特殊環状ペプチドを診断ツールとする低侵襲的な腫瘍特性解析法の開発」研究成果報告書　課題番号18K15306\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K15306/18K15306seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00057534","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学新学術創成研究機構ナノ生命科学研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=20781173"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=20781173","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K15306/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K15306/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K15306/18K15306seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K15306/18K15306seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-02-10"}],"displaytype":"detail","filename":"NA-PR-SATO-H-kaken 2021-7p.pdf","filesize":[{"value":"653.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"NA-PR-SATO-H-kaken 2021-7p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/51231/files/NA-PR-SATO-H-kaken 2021-7p.pdf"},"version_id":"650f147d-3701-40d6-9b9f-9d99f34afff5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"特殊環状ペプチドを診断ツールとする低侵襲的な腫瘍特性解析法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"特殊環状ペプチドを診断ツールとする低侵襲的な腫瘍特性解析法の開発"},{"subitem_title":"Development of a low-invasive diagnostic method for tumor characterization using macrocyclic peptide","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4014"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-10"},"publish_date":"2022-02-10","publish_status":"0","recid":"51231","relation_version_is_last":true,"title":["特殊環状ペプチドを診断ツールとする低侵襲的な腫瘍特性解析法の開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:51:51.090179+00:00"}