{"created":"2023-07-27T06:55:26.651697+00:00","id":51297,"links":{},"metadata":{"_buckets":{"deposit":"fb4d83c7-b5b1-4687-9824-68d25ff06c0b"},"_deposit":{"created_by":18,"id":"51297","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"51297"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00051297","sets":["2812:2813:4014"]},"author_link":["302","79940"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-11","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"9p.","bibliographicVolumeNumber":"2018-04-01 - 2021-03-31","bibliographic_titles":[{"bibliographic_title":"令和2(2020)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2020 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"植物表層における病原菌との相互作用に関わるタンパク質を同定するため、シロイヌナズナの葉に赤かび病菌の胞子懸濁液を滴下接種した表皮組織を用いて、ショットガンプロテオーム解析を行った。接種4時間後の表皮において、MAPKKK等のシグナル伝達因子やタンパク質分解に関わるタンパク質の発現量が増加していた。接種24時間後の表皮では、転写因子、PRタンパク質の発現も増加していたが、葉全体ではこれらの発現増加は見られなかった。また、赤かび病菌のエフェクター様タンパク質も多く検出されたことから、接種表皮を用いたプロテオーム解析は、植物と病原菌の相互作用に関わるタンパク質の検出に有効であることが示された。","subitem_description_type":"Abstract"},{"subitem_description":"The plant epidermis is the first line of plant defence against pathogen invasion and likely contains important regulatory proteins related to the plant-pathogen interaction. We performed comparative proteomic studies to identify differentially expressed proteins in the leaf epidermis challenged with fungal phytopathogen. The conidia solutions were dropped onto the Arabidopsis leaf surface and then collect the epidermal tissues from inoculated and mock treated leaves at 4 and 24 hpi. Label free quantification methods showed that proteins related to defence signaling, transcription factors, and cell wall organization were up-regulated in the inoculated leaf epidermis. More than 300 fungal proteins expressed on the Arabidopsis epidermis were also identified and about 13% of them were predicted as effector-like proteins. These results suggested that our method is useful for understanding the early expressions of plant and fungal proteins related to their interactions.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18K05642, 研究期間(年度):2018-04-01 - 2021-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「植物表皮プロテオミクスを用いた病原菌に対する侵入抵抗性の制御因子の探索と機能解析」研究成果報告書　課題番号18K05642\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K05642/18K05642seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学学際科学実験センター遺伝子研究施設","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00057600","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=20334790"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=20334790","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K05642/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K05642/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K05642/18K05642seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K05642/18K05642seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-02-10"}],"displaytype":"detail","filename":"AC-PR-NISHIUCHI-T-kaken 2021-9p.pdf","filesize":[{"value":"190.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"AC-PR-NISHIUCHI-T-kaken 2021-9p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/51297/files/AC-PR-NISHIUCHI-T-kaken 2021-9p.pdf"},"version_id":"c0f55cca-41a6-4fbe-a0be-50557adc31be"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"植物表皮プロテオミクスを用いた病原菌に対する侵入抵抗性の制御因子の探索と機能解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"植物表皮プロテオミクスを用いた病原菌に対する侵入抵抗性の制御因子の探索と機能解析"},{"subitem_title":"Proteomic study of plant epidermal tissues in response to phytopathogen invasion","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4014"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-10"},"publish_date":"2022-02-10","publish_status":"0","recid":"51297","relation_version_is_last":true,"title":["植物表皮プロテオミクスを用いた病原菌に対する侵入抵抗性の制御因子の探索と機能解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:51:24.930260+00:00"}