{"created":"2023-07-27T06:55:30.794290+00:00","id":51493,"links":{},"metadata":{"_buckets":{"deposit":"a3145c54-2c3b-4262-bbed-ffba42dccf36"},"_deposit":{"created_by":18,"id":"51493","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"51493"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00051493","sets":["2812:2813:4014"]},"author_link":["85","85740"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"18p.","bibliographicVolumeNumber":"2018-04-01 - 2021-03-31","bibliographic_titles":[{"bibliographic_title":"令和2(2020)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"},{"bibliographic_title":"2020 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究代表者がこれまでに収集してきたヒト乳がん臨床検体由来patient-derived xenograft (PDX)を活用し、ゲノム遺伝子変異の蓄積によるがん組織の不均一性と、幹細胞性を維持するシグナルを指標とした機能的ヒエラルキーを統合させる研究を行った。シングルセルRNAシークエンスより、増殖能が低い親玉がん幹細胞様細胞がクラスターを形成する遺伝子の中から、親玉がん幹細胞と通常のがん幹細胞を区別できる膜タンパク質FXYD3が見いだされた。エクソームシークエンスより、がん幹細胞様細胞に濃縮されやすい遺伝子変異がこれまで見逃されてきたことを強く示唆している。","subitem_description_type":"Abstract"},{"subitem_description":"To integrate the heterogeneity in cancer tissues due to accumulation of multiple mutations in genome and functional hierarchy due to different differentiation states in cancer cells, we analyzed breast cancer patient-derived xenograft models and spheroid cells. Single cell RNA sequencing following clustering analysis revealed that there are at least 2 clusters: proliferating cell cluster and non-proliferating cell cluster. We found a membrane protein FXYD3 among the highly expressing genes in the latter cell cluster and demonstrate that expression of FXYD3 is useful for distinguish the 2 populations. Furthermore, we discover novel mutations in genes that are not identified by bulk tissue analysis in cancer stem cell populations.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18H02679, 研究期間(年度):2018-04-01 - 2021-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「乳がんゲノム遺伝子変異と幹細胞性に基づく不均一性および階層性の統合解明」研究成果報告書　課題番号18H02679\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18H02679/18H02679seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん進展制御研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00057796","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10251448"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10251448","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02679/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02679/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18H02679/18H02679seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18H02679/18H02679seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-02-10"}],"displaytype":"detail","filename":"CA-PR-GOTOH-N-kaken 2021-18p.pdf","filesize":[{"value":"129.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-GOTOH-N-kaken 2021-18p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/51493/files/CA-PR-GOTOH-N-kaken 2021-18p.pdf"},"version_id":"0a61216b-d1d2-43d2-be26-3bd4417e269e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"乳がんゲノム遺伝子変異と幹細胞性に基づく不均一性および階層性の統合解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"乳がんゲノム遺伝子変異と幹細胞性に基づく不均一性および階層性の統合解明"},{"subitem_title":"Integrative analysis of genetical mutations and heterogeneity based on stemness in breast cancer cells","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4014"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-10"},"publish_date":"2022-02-10","publish_status":"0","recid":"51493","relation_version_is_last":true,"title":["乳がんゲノム遺伝子変異と幹細胞性に基づく不均一性および階層性の統合解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:50:54.097741+00:00"}