{"created":"2023-07-27T06:55:31.704447+00:00","id":51517,"links":{},"metadata":{"_buckets":{"deposit":"4e097fc2-402a-4341-bbd6-84820b95dc1e"},"_deposit":{"created_by":18,"id":"51517","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"51517"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00051517","sets":["2812:2813:4085"]},"author_link":["2838","25991"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-05-12","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2019-04-01 - 2022-03-31","bibliographic_titles":[{"bibliographic_title":"令和3(2021)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2021 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA repair disorders show the vulnerability by cytokine stimulations and induces apoptosis. Therefore, the establishment of novel transduction methodology, cytokine depletion method, was warranted. Baboon envelope pseudotyped lentiviral vector (Ba-LV) transduce without cytokine transduction, therefore we planed gene transfer using Ba-LV to Bloom syndrome model mouse (Blm), which is one of the representative DNA repair disorder. However, the efficacy of virus production was very low. Therefore, we succeeded to establish novel Ba-LV production protocol.\nThereafter, we verified vulnerability difference between wild type mouse's bone marrow cells and those of Blm by evaluating sister chromatid exchange. In the future, we would transduce Blm bone marrow cells with Ba-LV for recovering their function.","subitem_description_type":"Abstract"},{"subitem_description":"DNA修復障害疾患は、遺伝子導入の際に必要なサイトカイン刺激により細胞死が誘導される。そのため、サイトカイン刺激を省いた新規遺伝子導入法の開発が求められる。Baboonエンベロープを用いたレンチウイルス（Ba-LV）はサイトカイン刺激を省くことが可能であるため、それを用いてDNA修復障害の代表的疾患であるBloom症候群モデルマウス（Blm）を用いて遺伝子導入を行う。しかし、Ba-LVの産生効率が非常に悪いことが明らかとなり、新規ウイルス産生方法を樹立した。\n次に、Blmより採取した骨髄細胞の脆弱性確認を行ったところ野生型との有意な差異を確認できた。今後、Ba-LVを用いた機能回復実験を行う。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:19K07750, 研究期間(年度):2019-04-01 - 2022-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「エンベロープ改変レンチウイルスを用いた新規遺伝子治療前臨床試験モデルの樹立」課題番号19K07750\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K07750/19K07750seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学附属病院小児科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00057820","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10722043"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10722043","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K07750/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K07750/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K07750/19K07750seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K07750/19K07750seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-03"}],"displaytype":"detail","filename":"ME-PR-IKAWA-Y-kaken 2022-4p.pdf","filesize":[{"value":"141.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-IKAWA-Y-kaken 2022-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/51517/files/ME-PR-IKAWA-Y-kaken 2022-4p.pdf"},"version_id":"6eab6c61-cfbd-47ad-a67b-b4e67a934284"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"エンベロープ改変レンチウイルスを用いた新規遺伝子治療前臨床試験モデルの樹立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"エンベロープ改変レンチウイルスを用いた新規遺伝子治療前臨床試験モデルの樹立"},{"subitem_title":"Establishment of novel gene therapy preclinical model using Baboon envelope pseudotyped lentiviral vector","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4085"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-03"},"publish_date":"2023-02-03","publish_status":"0","recid":"51517","relation_version_is_last":true,"title":["エンベロープ改変レンチウイルスを用いた新規遺伝子治療前臨床試験モデルの樹立"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:38:33.476844+00:00"}