{"created":"2023-07-27T06:55:32.208116+00:00","id":51529,"links":{},"metadata":{"_buckets":{"deposit":"3f8b427b-debb-458f-b572-a0e2ed525e35"},"_deposit":{"created_by":18,"id":"51529","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"51529"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00051529","sets":["2812:2813:4014"]},"author_link":["190","85541"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-04-27","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"8p.","bibliographicVolumeNumber":"2018-06-29 – 2021-03-31","bibliographic_titles":[{"bibliographic_title":"令和2(2020)年度 科学研究費補助金 挑戦的研究(萌芽) 研究成果報告書"},{"bibliographic_title":"2020 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"脳回の獲得は脳機能の発達の基盤であると考えられており、従ってその形成メカニズムの解明は神経科学の重要研究課題の一つである。本研究では、発達した脳回をもつフェレットを用いて脳回形成機構の解明を目指した。ヒト滑脳症の原因遺伝子として知られているCdk5遺伝子のノックアウトを行った。Cdk5に対するCRISPR/Cas9プラスミドを導入した結果、神経細胞の放射状移動が障害され、さらに脳回の低形成が観察された。これらの結果から、神経細胞の放射状移動が脳回形成に必須であることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Folds in the cerebral cortex in mammals are believed to be key structures for accommodating increased cortical neurons in the cranial cavity. However, the mechanisms underlying cortical folding remain largely unknown. By combining in utero electroporation and the CRISPR/Cas9 system, we succeeded in efficient gene knockout of Cdk5, which is mutated in some patients with classical lissencephaly, in the gyrencephalic brains of ferrets. We show that Cdk5 knockout in the ferret cerebral cortex markedly impaired cortical folding. Furthermore, the results obtained from the introduction of dominant-negative Cdk5 into specific cortical layers suggest that Cdk5 function in upper-layer neurons is more important for cortical folding than that in lower-layer neurons. Cdk5 inhibition induced severe migration defects in cortical neurons. Taken together, our findings suggest that the appropriate positioning of upper-layer neurons is critical for cortical folding.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18K19376, 研究期間(年度):2018-06-29 – 2021-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「フェレットの大脳皮質形成機構の解明とその破綻による疾患病態の解析」研究成果報告書　課題番号18K19376\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K19376/18K19376seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00057832","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00418831"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00418831","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K19376/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K19376/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K19376/18K19376seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K19376/18K19376seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-02-03"}],"displaytype":"detail","filename":"ME-PR-SHINMYO-Y-kaken 2021-8p.pdf","filesize":[{"value":"312.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SHINMYO-Y-kaken 2021-8p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/51529/files/ME-PR-SHINMYO-Y-kaken 2021-8p.pdf"},"version_id":"963894c7-68c3-4b35-9f06-369505a60816"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"フェレットの大脳皮質形成機構の解明とその破綻による疾患病態の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"フェレットの大脳皮質形成機構の解明とその破綻による疾患病態の解析"},{"subitem_title":"Developmental mechanisms of the cerebral cortex in ferrets","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4014"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-03"},"publish_date":"2022-02-03","publish_status":"0","recid":"51529","relation_version_is_last":true,"title":["フェレットの大脳皮質形成機構の解明とその破綻による疾患病態の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:53:21.372882+00:00"}