{"created":"2023-07-27T06:55:45.926067+00:00","id":51974,"links":{},"metadata":{"_buckets":{"deposit":"40719d4e-78bb-47a2-928a-b63eb6d6e295"},"_deposit":{"created_by":18,"id":"51974","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"51974"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00051974","sets":["2812:2813:4014"]},"author_link":["2345","80537"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-08","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"8p.","bibliographicVolumeNumber":"2019-06-28 – 2021-03-31","bibliographic_titles":[{"bibliographic_title":"令和2(2020)年度 科学研究費補助金 挑戦的研究(萌芽) 研究成果報告書"},{"bibliographic_title":"2020 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"棘皮動物は左右相称動物に属すが、幼生内に成体原基を形成し、五放射の成体へと変態する。しかし、成体原基形成の分子メカニズムはほとんど分かっていない。\nCRISPR/Cas9システムを用いてfgf8/17/18とfgfaをノックアウトしたウニ幼生の成体原基の表現型を解析し、以下のことを明らかにした。（1）羊膜外胚葉で自律的に発現するfgf8/17/18が原腸から水腔中胚葉を誘導し、水腔からの放射水管伸長とそれを覆う歩帯外胚葉特異化を調節している。（2）fgf8/17/18は水腔fgfaを誘導するが、放射線（歩帯）の確立は、fgf8/17/18下流にある未同定のシグナルによって調節されるらしい。","subitem_description_type":"Abstract"},{"subitem_description":"Echinoderms belong to bilaterian animals, but they form the adult rudiment in larvae and metamorphose into pentaradial juveniles. However, little is known about the molecular mechanisms underlying the adult rudiment formation.\nUsing CRISPR/Cas9 system, I knocked out fgf8/17/18 and fgfa in sea urchin larvae and analyzed phenotypes of the adult rudiment from both the three-dimensional images and the expression of marker genes, and clarified the followings. (1) fgf8/ 7/18 autonomously expressed in the vestibule ectoderm induces the hydrocoel mesoderm from the archenteron and regulates the extension of radial canals from the hydrocoel. Therefore, fgf8/ 7/18 KO larvae fails to form adult rudiment and cannot metamorphose, (2) fgf8/17/18 activates fgfa in the hydrocoel, but fgfa KO larvae can metamorphose into pentaradial juveniles, and (3) establishment of echinoderm’s rays is likely regulated by unidentified signals downstream of fgf8 /17/18.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:19K22420, 研究期間(年度):2019-06-28 – 2021-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「棘皮動物の五放射体制はどのように進化したのか -放射線を確立する分子機構の解明-」研究成果報告書　課題番号19K2242\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-19K22420/19K22420seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域生命理工学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00058274","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=60182458"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=60182458","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22420/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22420/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-19K22420/19K22420seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-19K22420/19K22420seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-02-03"}],"displaytype":"detail","filename":"SC-PR-YAMAGUCHI-M-kaken 2021-8p.pdf","filesize":[{"value":"663.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-YAMAGUCHI-M-kaken 2021-8p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/51974/files/SC-PR-YAMAGUCHI-M-kaken 2021-8p.pdf"},"version_id":"5094fb42-8b2c-4e34-856f-a08406206fd3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"棘皮動物の五放射体制はどのように進化したのか: 放射線を確立する分子機構の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"棘皮動物の五放射体制はどのように進化したのか: 放射線を確立する分子機構の解明"},{"subitem_title":"Evolution of echinoderms -molecular mechanisms that establish the rays-","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4014"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-03"},"publish_date":"2022-02-03","publish_status":"0","recid":"51974","relation_version_is_last":true,"title":["棘皮動物の五放射体制はどのように進化したのか: 放射線を確立する分子機構の解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:53:11.882557+00:00"}