{"created":"2023-07-27T06:55:46.152344+00:00","id":52001,"links":{},"metadata":{"_buckets":{"deposit":"09ca2713-ed5b-41fc-a015-a86aa4633fb7"},"_deposit":{"created_by":18,"id":"52001","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"52001"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00052001","sets":["2812:2813:4085"]},"author_link":["3006","27243"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2020-04-01 – 2022-03-31","bibliographic_titles":[{"bibliographic_title":"令和3(2021)年度 科学研究費補助金 若手研究 研究成果報告書"},{"bibliographic_title":"2021 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Arakawa, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"27243","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40709028","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=40709028"},{"nameIdentifier":"40709028","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000040709028"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present study aimed at identifying drug uptake transporter in blood-brain barrier (BBB) model hCMEC/D3 cells using a knockdown/knockout screening study targeting membrane transporters, including uncharacterized ones. The reported and estimated unbound brain to plasma concentration ratio values of 65 kinds of drugs were investigated, and 10 drugs, which exhibited higher Kp,uu,brain, were selected for following screenings. According to studies of siRNA knockdown screening in hCMEC/D3 cells, OCTN2 was identified as candidate transporter gene for aripiprazole. On the other hand, by the knockout screening with the consideration of the expression of extracted genes in human BBB, 10 genes were found as BBB uptake transporter candidates. In conclusion, knockdown and knockout screening are useful approaches for identifying BBB transporters.","subitem_description_type":"Abstract"},{"subitem_description":"本研究では、血液脳関門における薬物トランスポーターを同定することを目的とした。Kp,uu,brain値が1を超える薬物を文献調査から抽出し、これら薬物のhCMEC/D3細胞における温度依存的な取り込み試験を行い、トランスポーター介在輸送が期待される薬物を絞り込んだ。さらに、輸送体標的型siRNAライブラリーを用いたスクリーニング試験を行い、OCTN2がアリピプラゾールの脳移行に関与する取り込み輸送体であることが示された。推定された輸送体のみに検討を絞った従来の限定的な手法と比べ、本手法は未知の薬物輸送体を同定する手法として有用であることが示唆された。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:20K16039, 研究期間(年度):2020-04-01 – 2022-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「sgRNAライブラリーを用いた血液脳関門輸送体の同定」課題番号20K16039\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-20K16039/20K16039seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00058301","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40709028"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40709028","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K16039/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K16039/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-20K16039/20K16039seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-20K16039/20K16039seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"荒川, 大 "}],"nameIdentifiers":[{"nameIdentifier":"3006","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40709028","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40709028"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-09"}],"displaytype":"detail","filename":"PH-PR-ARAKAWA-H-kaken 2022-4p.pdf","filesize":[{"value":"139.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-ARAKAWA-H-kaken 2022-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/52001/files/PH-PR-ARAKAWA-H-kaken 2022-4p.pdf"},"version_id":"e193ed2a-4319-4f9c-bcea-f09c26e47501"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"sgRNAライブラリーを用いた血液脳関門輸送体の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"sgRNAライブラリーを用いた血液脳関門輸送体の同定"},{"subitem_title":"Identification of blood-brain barrier transporters using sgRNA library","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4085"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-09"},"publish_date":"2023-02-09","publish_status":"0","recid":"52001","relation_version_is_last":true,"title":["sgRNAライブラリーを用いた血液脳関門輸送体の同定"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:16:00.285045+00:00"}