{"created":"2023-07-27T06:55:48.703710+00:00","id":52079,"links":{},"metadata":{"_buckets":{"deposit":"db1ea369-8d70-4f9a-b0e1-97c0d80c50f6"},"_deposit":{"created_by":18,"id":"52079","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"52079"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00052079","sets":["2812:2813:4085"]},"author_link":["366","80108"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-06","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"8p.","bibliographicVolumeNumber":"2019-06-28 – 2022-03-31","bibliographic_titles":[{"bibliographic_title":"令和3(2021)年度 科学研究費補助金 挑戦的研究(萌芽) 研究成果報告書"},{"bibliographic_title":"2021 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have developed \"nanoendoscopy\" that allows to visualize nanodynamics inside living cells based on atomic force microscopy. This technology visualizes intracellular structures by scanning a needle tip in three-dimensional space, including inside the cell, and recording the forces acting on the tip during the scanning. Furthermore, by inserting the needle into the cell and performing AFM observation there, the local intracellular structure and its dynamics can be observed with nano-level resolution. In addition, it was confirmed by fluorescence assay that these measurements do not cause lethal damage to the cells. This technology enables direct observation of intracellular phenomena that could not be observed previously, and is expected to advance our understanding of the mechanisms underlying the expression of cellular functions.","subitem_description_type":"Abstract"},{"subitem_description":"生細胞内部でナノ構造・動態を計測できるナノ内視鏡技術を、原子間力顕微鏡（AFM)を基盤として開発した。この技術では、ニードル探針を細胞内を含む3次元空間内で走査し、その間に探針に働く力を記録することで細胞内構造を可視化する。さらに、細胞内に探針を挿入し、そこでAFM観察を行うことで、細胞内の局所構造やその動態を、ナノレベルの分解能で観察できる。また、これらの計測が、細胞に致死的なダメ－ジを与えないことを蛍光アッセイにより確認した。この技術により、従来観察できなかった細胞内現象を直接観ることが可能となり、細胞機能の発現機構に関する理解が進むものと期待される。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:19K22125, 研究期間(年度):2019-06-28 – 2022-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「生きた細胞内部のナノ動態を直接可視化するナノ内視鏡技術の開発」課題番号19K22125\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K22125/19K22125seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学ナノ生命科学研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00058379","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90452094"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90452094","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22125/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22125/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K22125/19K22125seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K22125/19K22125seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-10"}],"displaytype":"detail","filename":"NAL-PR-FUKUMA-T-kaken 2022-8p.pdf","filesize":[{"value":"816.7 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"NAL-PR-FUKUMA-T-kaken 2022-8p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/52079/files/NAL-PR-FUKUMA-T-kaken 2022-8p.pdf"},"version_id":"e14b0447-b96d-43a9-b48a-d8b28bbd8b38"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"生きた細胞内部のナノ動態を直接可視化するナノ内視鏡技術の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"生きた細胞内部のナノ動態を直接可視化するナノ内視鏡技術の開発"},{"subitem_title":"Development of nanoendoscopy techinique for direct visualization of nanodynamics inside living cells","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4085"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-10"},"publish_date":"2023-02-10","publish_status":"0","recid":"52079","relation_version_is_last":true,"title":["生きた細胞内部のナノ動態を直接可視化するナノ内視鏡技術の開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:35:51.010945+00:00"}