{"created":"2023-07-27T06:55:55.639580+00:00","id":52410,"links":{},"metadata":{"_buckets":{"deposit":"1aa53a04-2b2a-416d-8da0-a13d7371b4c8"},"_deposit":{"created_by":18,"id":"52410","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"52410"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00052410","sets":["2812:2813:2818"]},"author_link":["93682"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-04-10","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6p.","bibliographicVolumeNumber":"2012-04-01 – 2016-03-31","bibliographic_titles":[{"bibliographic_title":"平成27(2015)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2015 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"解糖系は重要な代謝システムであるがその制御の全容は明らかでない。そこで、本研究では新規解糖系制御遺伝子を探索するためゲノムワイドshRNAライブラリーを用いたスクリーニングを実施し、5種類の遺伝子を同定した。その中でRNF126について詳細な解析を行った結果、RNF126はPDKのユビキチンリガーゼとして働くことで、ピルビン酸からアセチルCoAへの変換が促進することがわかった。RNF126欠損により軟寒天培地のコロニー形成やマウスでの造腫瘍能が抑制された。さらに、RNF126はがんのanoikis耐性に関わるERKシグナルにより発現誘導がかかることが明らかとなった。","subitem_description_type":"Abstract"},{"subitem_description":"Glycolysis is a vital metabolic system. However, our understanding on glycolysis remains limited. To identify new genes involved in glycolysis regulation, we have performed a genome-wide gene knockdown analysis in human lung carcinoma PC8 cells using a mitochondrial inhibitor oligomycin and an shRNA library carried by a lentiviral vector, and finally identified five genes. Among them, we further focused on RNF126. RNF126 was found to act as a ubiquitin ligase for PDKs, resulting in their proteasomal degradation. This decrease in PDK levels allowed pyruvate dehydrogenases to catalyze the conversion of pyruvate to acetyl CoA. Moreover, depletion of RNF126 in cancer cells suppressed colony formation in soft agar as well as tumorigenicity in mice. RNF126 expression was found to be under the control of the ERK signaling pathway, which is essential for anoikis resistance. Thus, RNF126 is an attractive molecule for treating cancer by selectively targeting anchorage-independent growth.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:24591313, 研究期間(年度):2012-04-01 – 2016-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「合成致死性を応用した解糖系制御遺伝子の効率的網羅的探索」課題番号24591313\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-24591313/24591313seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系 / 東京大学","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00058704","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=70511418"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=70511418","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-24591313/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-24591313/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-24591313/24591313seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-24591313/24591313seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-17"}],"displaytype":"detail","filename":"ME-PR-SAKAMOTO-T-kaken 2016-6p.pdf","filesize":[{"value":"172.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SAKAMOTO-T-kaken 2016-6p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/52410/files/ME-PR-SAKAMOTO-T-kaken 2016-6p.pdf"},"version_id":"de6835a3-2f88-49e9-a1ed-90a93d4caeea"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"合成致死性を応用した解糖系制御遺伝子の効率的網羅的探索","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"合成致死性を応用した解糖系制御遺伝子の効率的網羅的探索"},{"subitem_title":"Comprehensive screening of glycolysis-related genes by synthetic lethality","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2818"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-07-17"},"publish_date":"2020-07-17","publish_status":"0","recid":"52410","relation_version_is_last":true,"title":["合成致死性を応用した解糖系制御遺伝子の効率的網羅的探索"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:50:35.859216+00:00"}