{"created":"2023-07-27T06:56:00.556979+00:00","id":52540,"links":{},"metadata":{"_buckets":{"deposit":"07386861-83b3-4177-aded-6326f1ed52f3"},"_deposit":{"created_by":18,"id":"52540","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"52540"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00052540","sets":["2812:2813:2818"]},"author_link":["94105"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-06-13","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2014-04-01 – 2016-03-31","bibliographic_titles":[{"bibliographic_title":"平成27(2015)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2015 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sumino, Ayumi"}],"nameIdentifiers":[{"nameIdentifier":"94105","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80717140","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80717140"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"pH依存性カリウムチャネルKcsAの構造は、大きく分けて膜貫通ドメインと細胞質ドメインに分けられる。膜貫通ドメインの高分解構造は結晶構造により明らかになっているが、細胞質ドメインの高分解構造は不明である。本研究では、水中・非結晶化状態におけるKcsAチャネルの細胞質ドメインの構造および構造変化の端緒をつかむことを目的とした。原子間力顕微鏡観察により、水中においてはKcsAチャネルの細胞質ドメインは高速に揺らいでおり、硬い特定の構造をとらないことを明らかにした。蛍光色素を用いた実験により、閉状態から開状態へ変化する際に、細胞質ドメイン末端同士の距離が離れることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Structure of pH-dependent potassium channel KcsA is roughly divided into transmembrane and cytoplasmic domains. The high resolution structure of the transmembrane domains have been revealed by the crystal structure, but the high resolution structure of the cytoplasmic domain is unknown. In this study, we aimed to elucidate the structure of the cytoplasmic domain of KcsA channel in water and non-crystalline state. Atomic force microscopy revealed that, the cytoplasmic domain of KcsA channel were fluctuating at a high speed. The result indicates that the cytoplasmic domain of KcsA does not take a hard specific structure in water.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26870233, 研究期間(年度):2014-04-01 – 2016-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「高分解顕微鏡観察を主としたカリウムチャネルKcsAの柔軟な構造と機能の相関解明」課題番号26870233\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-26870233/26870233seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学新学術創成研究機構 / 福井大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00058834","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=80717140"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=80717140","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-26870233/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-26870233/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-26870233/26870233seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-26870233/26870233seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"角野, 歩"}],"nameIdentifiers":[{"nameIdentifier":"94105","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80717140","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80717140"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-10"}],"displaytype":"detail","filename":"FR-PR-SUMINO-A-kaken 2016-4p.pdf","filesize":[{"value":"165.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"FR-PR-SUMINO-A-kaken 2016-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/52540/files/FR-PR-SUMINO-A-kaken 2016-4p.pdf"},"version_id":"58b2f618-da65-41a4-8546-1540af2eaa7e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"高分解顕微鏡観察を主としたカリウムチャネルKcsAの柔軟な構造と機能の相関解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高分解顕微鏡観察を主としたカリウムチャネルKcsAの柔軟な構造と機能の相関解明"},{"subitem_title":"Elucidation of the flexible structure-function relationship of the potassium channel KcsA using high resolution microscopy","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2818"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-09-10"},"publish_date":"2020-09-10","publish_status":"0","recid":"52540","relation_version_is_last":true,"title":["高分解顕微鏡観察を主としたカリウムチャネルKcsAの柔軟な構造と機能の相関解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:41:29.196608+00:00"}