{"created":"2023-07-27T06:56:05.415159+00:00","id":52695,"links":{},"metadata":{"_buckets":{"deposit":"2b16a2be-183c-41c6-8c46-c73b9ad3df9e"},"_deposit":{"created_by":18,"id":"52695","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"52695"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00052695","sets":["2812:2813:4014"]},"author_link":["94345"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-04-20","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2019-08-30 – 2021-03-31","bibliographic_titles":[{"bibliographic_title":"令和2(2020)年度 科学研究費補助金 研究活動スタート支援 研究成果報告書"},{"bibliographic_title":"2020 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wang, Dong"}],"nameIdentifiers":[{"nameIdentifier":"94345","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20842983","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20842983"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"アクチビンと大腸がんドライバー遺伝子変異の相互作用が、細胞のアポトーシスとEMT誘導の関与について検討した。様々なドライバー遺伝子変異を持つオルガノイドをアクチビンで刺激した結果、Kras変異がアクチビン誘導アポトーシスを抑制すること、さらに、アクチビンが変異型p53遺伝子との相互作用でEMTを誘導することがわかった。また、アクチビンで刺激したオルガノイドの移植は、刺激してないオルガノイドより多くの肺転移巣を形成した。RNA-seqの結果から、アクチビンと変異型p53遺伝子で、いくつかのシグナル経路が誘導されることがわかった。これらの結果から、新たな候補阻害剤を検索できる可能性を示唆した。","subitem_description_type":"Abstract"},{"subitem_description":"In order to know the role of activin in colorectal cancer EMT and which driver gene mutations contribute to activin's conversion from induction of apoptosis to induction of EMT, the organoids with different combination of driver gene mutations were simulated with activin and the cell survival and EMT were examined. Cell survival: by using activin simulation and blocking Kras-MEK signaling, we found that Kras mutation inhibits activin-induced apoptosis. Morphological changes: we found that activin induces EMT via interacting with p53 homozygous mutation. In vivo: activin pretreated organoids form more lung metastasis. For mechanism: by using RNA-seq, we found that under the regulation of activin and p53 homozygous mutation, some signaling pathways, such as Wnt, p38-MAPK, Stat3 signaling, were upregulated. According to these findings we will be able to test some inhibitors for inhibiting the morphological changes and the candidate inhibitors may be used for clinical trial.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:19K23882, 研究期間(年度):2019-08-30 – 2021-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「The role of activin signaling in colorectal cancer EMT induction」研究成果報告書　課題番号19K23882\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K23882/19K23882seika/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学ナノ生命科学研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00058989","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=20842983"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=20842983","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19K23882/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19K23882/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K23882/19K23882seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K23882/19K23882seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wong, Dong"}],"nameIdentifiers":[{"nameIdentifier":"94345","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20842983","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20842983"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-02-25"}],"displaytype":"detail","filename":"FR-PR-WANG-D-kaken 2021-4p.pdf","filesize":[{"value":"621.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"FR-PR-WANG-D-kaken 2021-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/52695/files/FR-PR-WANG-D-kaken 2021-4p.pdf"},"version_id":"67339587-f36c-4596-ac46-1e12c5dadaf9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"The role of activin signaling in colorectal cancer EMT induction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The role of activin signaling in colorectal cancer EMT induction"},{"subitem_title":"The role of activin signaling in colorectal cancer EMT induction","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4014"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-25"},"publish_date":"2022-02-25","publish_status":"0","recid":"52695","relation_version_is_last":true,"title":["The role of activin signaling in colorectal cancer EMT induction"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:19:31.507825+00:00"}