{"created":"2023-07-27T06:56:08.005773+00:00","id":52787,"links":{},"metadata":{"_buckets":{"deposit":"917c9d2a-496b-4d79-bfb3-5599e50aa53b"},"_deposit":{"created_by":18,"id":"52787","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"52787"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00052787","sets":["2812:2813:4014"]},"author_link":["94588"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-08","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2018-04-01 – 2021-03-31","bibliographic_titles":[{"bibliographic_title":"令和2(2020)年度 科学研究費補助金 若手研究 研究成果報告書"},{"bibliographic_title":"2020 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kokusho, Ryuhei"}],"nameIdentifiers":[{"nameIdentifier":"94588","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90756537","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90756537"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"申請者は、カイコ核多角体病ウイルス（BmNPV）が宿主昆虫であるカイコ幼虫の脳に感染することで、カイコの行動を操っていると着想した。そこで、本研究ではGAL4/UASシステムを用いることで、カイコの中枢神経系のみでウイルス増殖を抑制できる遺伝子組換えカイコを作出することで、この仮説の実証を試みた。結果として、ウイルス増殖を大幅に抑制できるカイコ系統は作出できたが、その効果を中枢神経系のみに限定するカイコ系統の作出に難航している。","subitem_description_type":"Abstract"},{"subitem_description":"We hypothesized that baculoviruses manipulate host behavior by directly infecting and controlling host central nervous system (CNS). To demonstrate this, we have tried to generate GAL4/UAS transgenic silkworm strains that can suppress viral propagation in specific tissues including CNS. We obtained UAS strains, which express CRISPR-Cas9 system for the cleavage of viral DNA. In the F1 hybrid of these UAS strains and the ActinA3-GAL4 strain, viral propagation significantly delayed. Also, we tried to generate CNS-specific GAL4 strains by GAL4 knock-in into neuron-specific gene loci, but no strains can induce neuron-specific expression so far.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18K14471, 研究期間(年度):2018-04-01 – 2021-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「ウイルスは宿主の脳を操るか？：脳特異的ウイルス制御機構を用いた脳機能操作の実証」研究成果報告書　課題番号18K14471\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18K14471/18K14471seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域生命理工学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00059080","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=90756537"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=90756537","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18K14471/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18K14471/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18K14471/18K14471seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18K14471/18K14471seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"國生, 龍平"}],"nameIdentifiers":[{"nameIdentifier":"94588","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90756537","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90756537"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-03"}],"displaytype":"detail","filename":"SC-PR-KOKUSYO-R-kaken 2021-4p.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-KOKUSYO-R-kaken 2021-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/52787/files/SC-PR-KOKUSYO-R-kaken 2021-4p.pdf"},"version_id":"e4784966-2d96-49a6-ab93-d3a3cd33c73a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ウイルスは宿主の脳を操るか？: 脳特異的ウイルス制御機構を用いた脳機能操作の実証","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ウイルスは宿主の脳を操るか？: 脳特異的ウイルス制御機構を用いた脳機能操作の実証"},{"subitem_title":"How baculoviruses manipulate host brain?: realationship between neuronal infection and host manipulation","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["4014"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-03"},"publish_date":"2022-03-03","publish_status":"0","recid":"52787","relation_version_is_last":true,"title":["ウイルスは宿主の脳を操るか？: 脳特異的ウイルス制御機構を用いた脳機能操作の実証"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:19:29.324848+00:00"}