{"created":"2023-07-27T06:56:15.551744+00:00","id":53045,"links":{},"metadata":{"_buckets":{"deposit":"f2418435-473d-4bd6-b99b-624dfe74ac9d"},"_deposit":{"created_by":18,"id":"53045","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"53045"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00053045","sets":["2812:2813:2816"]},"author_link":["1898"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-06-06","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2016-04-01 – 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒト脳脊髄液のAmyloidβ蛋白に対するオリゴマー化抑制作用の原因物質の解明を行った。脳脊髄液を液体クロマトグラフィー(Size-exclusion column)で分注し、MS/MSで評価し、Angiotensin I、II、IIIの他、既知の蛋白の断片などの16種のペプチドの合成を行った。オリゴマー化の抑制力をPICUP法で評価し、AngiotensinI-IIIを含む11種のペプチドで抑制力をみとめ、Aβの線維化に対する抑制力をThioflavin T法を用いて評価し、9種のペプチドで抑制力を認めた。これらの蛋白はAlzheimer病の発生機序の究明、治療法の開発に寄与する。","subitem_description_type":"Abstract"},{"subitem_description":"The purpose of this research was to identify inhibitors of amyloid β (Aβ) oligomerization in human cerebrospinal fluid (CSF). CSF was fractionated using centrifugation through a column and size-exclusion high-performance liquid chromatography. Two CSF fractions had inhibitory effects on Aβ oligomerization, as determined using tandem mass spectrometry. We identified 16 peptides with the desired activity: Angiotensins I, II, and III, and known fragments of these peptides. Using photo-induced cross-linking of unmodified proteins (PICUP), we identified 11 peptides with Aβ oligomerization inhibitory activity, including Angiotensins I-III. Thioflavin T fluorescence was used to identify 9 peptides with Aβ fibrillation inhibitory activity, including Angiotensins I-III. The discovery of these peptides contributes to the investigation of the mechanism underlying Alzheimer's disease pathology and the development of therapeutic strategies for Alzheimer’s disease.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16K19506, 研究期間(年度):2016-04-01 – 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「脳脊髄液中のアミロイドβオリゴマー化抑制物質解明と早期診断・治療法開発の展開」課題番号16K19506\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K19506/16K19506seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00059329","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=30755773"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=30755773","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K19506/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K19506/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K19506/16K19506seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K19506/16K19506seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-19"}],"displaytype":"detail","filename":"ME-PR-IKEDA-T-kaken 2018-4p.pdf","filesize":[{"value":"281.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-IKEDA-T-kaken 2018-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/53045/files/ME-PR-IKEDA-T-kaken 2018-4p.pdf"},"version_id":"b3c991f3-e665-45f1-a78f-4186241d93fa"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"脳脊髄液中のアミロイドβオリゴマー化抑制物質解明と早期診断・治療法開発の展開","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"脳脊髄液中のアミロイドβオリゴマー化抑制物質解明と早期診断・治療法開発の展開"},{"subitem_title":"Inhibitors of amyloid beta oligomerization in human cerebrospinal fluid","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-10-19"},"publish_date":"2020-10-19","publish_status":"0","recid":"53045","relation_version_is_last":true,"title":["脳脊髄液中のアミロイドβオリゴマー化抑制物質解明と早期診断・治療法開発の展開"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:56:01.667589+00:00"}