{"created":"2023-07-27T06:56:15.777036+00:00","id":53051,"links":{},"metadata":{"_buckets":{"deposit":"b59522d8-d4f1-4e6f-b4b7-3947900dae16"},"_deposit":{"created_by":18,"id":"53051","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"53051"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00053051","sets":["2812:2813:2815"]},"author_link":["2744"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-18","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6p.","bibliographicVolumeNumber":"2017-04-01 – 2019-03-31","bibliographic_titles":[{"bibliographic_title":"平成30(2018)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2018 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Noshiro,  Daisuke"}],"nameIdentifiers":[{"nameIdentifier":"2744","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90751107","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90751107"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"六量体リングを形成し、二本鎖DNAを一本鎖へと分離するヘリカーゼとして機能する2種の古細菌由来MCM（ssoMCMとmthMCM）タンパク質を高速AFMにより観察した。マイカ上でのssoMCMタンパク質全長の観察の結果、ヘリカーゼ活性を持つと考えられている六量体リング（およびその部分構造）の他、梯子状に連なった長い複合体や六量体以上の多量体リング（およびその部分構造）の形成が確認された。また、タマビジン2の二次元結晶上に固定したビオチン化mthMCMタンパク質全長の観察の結果、横倒しの状態で固定された六量体リングおよびダブル六量体リング（十二量体）と思しき2種類の構造体の存在を確認した。","subitem_description_type":"Abstract"},{"subitem_description":"Two archaeal MCM (ssoMCM and mthMCM) proteins which have helicase activities to unwind double-stranded DNA to produce single-stranded DNA were observed by high-speed AFM (HS-AFM). The observation of the full-length ssoMCM protein on mica surface revealed the formation of ladder-like long-shaped oligomers and ring (or partial ring) structures composed of more than hexamer as well as hexameric ring structure with helicase activity (or partial hexameric ring structure). In addition, observation of biotinylated full-length mthMCM protein on the 2D crystals of tamavidin 2 confirmed the formation of hexameric ring and double hexameric ring (12 mer) structures.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:17K15433, 研究期間(年度):2017-04-01 – 2019-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「高速AFMによる古細菌由来MCMタンパク質複合体の観察」課題番号17K15433\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-17K15433/17K15433seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"微生物化学研究会 微生物化学研究所 / 金沢大学","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00059335","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=90751107"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=90751107","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17K15433/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17K15433/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-17K15433/17K15433seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-17K15433/17K15433seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"能代, 大輔 "}],"nameIdentifiers":[{"nameIdentifier":"2744","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90751107","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90751107"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-10"}],"displaytype":"detail","filename":"ME-PR-NOSHIRO-D-kaken 2019-6p.pdf","filesize":[{"value":"348.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-NOSHIRO-D-kaken 2019-6p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/53051/files/ME-PR-NOSHIRO-D-kaken 2019-6p.pdf"},"version_id":"4e795c4b-45e0-48ba-a1d1-3771cc2b0b70"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"高速AFMによる古細菌由来MCMタンパク質複合体の観察","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高速AFMによる古細菌由来MCMタンパク質複合体の観察"},{"subitem_title":"High-speed AFM observation of archaeal MCM protein complexes","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2815"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-09-10"},"publish_date":"2020-09-10","publish_status":"0","recid":"53051","relation_version_is_last":true,"title":["高速AFMによる古細菌由来MCMタンパク質複合体の観察"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:29:17.895721+00:00"}