{"created":"2023-07-27T06:56:16.541053+00:00","id":53069,"links":{},"metadata":{"_buckets":{"deposit":"43b37a8d-e4aa-45a1-810c-da8d81ac5a19"},"_deposit":{"created_by":18,"id":"53069","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"53069"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00053069","sets":["2812:2813:2816"]},"author_link":["72542","20207"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-31","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2015-04-01 – 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究の目的は、肥大型心筋症におけるホスホリラーゼ異常スレオニンリン酸化の機能的意義を明らかにし、新規心不全発症機序を解明することである。精製したWT-ホスホリラーゼ, ThrXAla-ホスホリラーゼ, ThrXAsp-ホスホリラーゼを用いて機能発現実験を行った。NADPH産生量を指標としたForward反応では、ThrXAsp-ホスホリラーゼ活性はWT-ホスホリラーゼおよびThrXAla-ホスホリラーゼ活性と比較して有意に低下した。ゲル泳動Reverse反応においても、Forward反応と同様にThrXAsp-ホスホリラーゼ活性低下が明らかとなった。","subitem_description_type":"Abstract"},{"subitem_description":"We aimed to investigate the role of phosphorylase in the development of heart failure. In vitro forward assay showed that WT-phosphorylase activity was significantly decreased compared with ThrXAla phosphorylase and ThrXAsp phosphorylase. This association was also observed in the reverse gel assay.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15K09134, 研究期間(年度):2015-04-01 – 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「ホスホリラーゼ異常リン酸化による心不全発症機序の解明」課題番号15K09134\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-15K09134/15K09134seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00059353","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=50377389"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=50377389","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-15K09134/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-15K09134/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-15K09134/15K09134seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-15K09134/15K09134seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-19"}],"displaytype":"detail","filename":"ME-PR-KONNO-T-kaken 2018-4p.pdf","filesize":[{"value":"75.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KONNO-T-kaken 2018-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/53069/files/ME-PR-KONNO-T-kaken 2018-4p.pdf"},"version_id":"5ab05eef-d45d-443f-8db4-47cf50fc7a7e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ホスホリラーゼ異常リン酸化による心不全発症機序の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ホスホリラーゼ異常リン酸化による心不全発症機序の解明"},{"subitem_title":"Phosphorylase phosphorylation in the heart failure development","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-10-19"},"publish_date":"2020-10-19","publish_status":"0","recid":"53069","relation_version_is_last":true,"title":["ホスホリラーゼ異常リン酸化による心不全発症機序の解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:56:07.771424+00:00"}