{"created":"2023-07-27T06:56:30.259482+00:00","id":53336,"links":{},"metadata":{"_buckets":{"deposit":"f3ed9b66-75c6-411b-9dbc-0550a73721b5"},"_deposit":{"created_by":18,"id":"53336","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"53336"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00053336","sets":["2812:2813:2820"]},"author_link":["21904","95221"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-05-27","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2011 – 2013","bibliographic_titles":[{"bibliographic_title":"平成25(2013)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2013 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"β1,4-ガラクトース転移酵素－2（β4GalT-2)遺伝子ノックアウトマウスの行動学的解析からβ4GalT-2が合成するガラクトース糖鎖が中枢神経系で機能を持つことが明らかとなった。そこで、β4GalT-2が主要な働きを持つ脳領域や細胞の特定を行い、個体から機能糖鎖分子に至る手がかりを得る為に新しいノックインマウスの作製を行うことにした。β4GalT-2遺伝子の発現をLacZ遺伝子によってモニターできるターゲティングベクターと、部位・時期特異的に機能を回復させることができる2種類のベクターを新しい方法を用いて構築を試み、難航したが構築が完了しES細胞に導入することができた。","subitem_description_type":"Abstract"},{"subitem_description":"From behavioral analyses of beta 1, 4-galactosyltransferase-2 (B4GalT-2) gene knockout mice, we have found that galactose sugar chains synthesized by B4GalT-2 have a function in the central nervous system. Therefore, we tried to generate novel knock-in mice in order to identify cells and brain regions where B4GalT-2 is involved, to obtain clues identifying functional carbohydrate molecules. We constructed two kinds of targeting vectors, one for monitoring B4GalT-2 expression by lacZ gene and the other for recovering B4GalT-2 expression conditionally. We are trying to isolate desired ES clones.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23500493, 研究期間(年度):2011 – 2013","subitem_description_type":"Other"},{"subitem_description":"出典：「糖転移酵素ノックアウトマウス表現型と糖鎖機能を解明する方法論の開発と実証」研究成果報告書　課題番号23500493\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-23500493/23500493seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00059620","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=10377418"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=10377418","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-23500493/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-23500493/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-23500493/23500493seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-23500493/23500493seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-05"}],"displaytype":"detail","filename":"ME-PR-SUGIHARA-K-kaken 2014-4p.pdf","filesize":[{"value":"511.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SUGIHARA-K-kaken 2014-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/53336/files/ME-PR-SUGIHARA-K-kaken 2014-4p.pdf"},"version_id":"a9d06df2-56a6-489f-871d-f517c0331dac"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"糖転移酵素ノックアウトマウス表現型と糖鎖機能を解明する方法論の開発と実証","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"糖転移酵素ノックアウトマウス表現型と糖鎖機能を解明する方法論の開発と実証"},{"subitem_title":"The development and demonstration of methodology to elucidate the sugar chain function and glycosyltransferase knockout mouse phenotype.","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2820"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-05"},"publish_date":"2020-11-05","publish_status":"0","recid":"53336","relation_version_is_last":true,"title":["糖転移酵素ノックアウトマウス表現型と糖鎖機能を解明する方法論の開発と実証"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T09:15:28.068949+00:00"}