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Nanoscale probing and visualization of cyclic nucleotide-gated ion channels structural dynamics and function
https://doi.org/10.24517/00059663
https://doi.org/10.24517/000596632347350b-87ec-4ff2-8243-4589831e439c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
NAL-PR-MARCHESI-A-kaken 2022-1p.pdf (84.0 kB)
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| Item type | 報告書 / Research Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2023-07-20 | |||||
| タイトル | ||||||
| タイトル | Nanoscale probing and visualization of cyclic nucleotide-gated ion channels structural dynamics and function | |||||
| タイトル | ||||||
| タイトル | Nanoscale probing and visualization of cyclic nucleotide-gated ion channels structural dynamics and function | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
| 資源タイプ | research report | |||||
| ID登録 | ||||||
| ID登録 | 10.24517/00059663 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
Marchesi, Arin
× Marchesi, Arin |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学ナノ生命科学研究所 | |||||
| 書誌情報 |
令和3(2021)年度 科学研究費補助金 研究活動スタート支援 研究課題概要 en : 2021 Research Project Summary 巻 2020-09-11 – 2022-03-31, p. 1p., 発行日 2022-12-28 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The proposal will advance our understanding of ion channels function by exploiting a recently developed mechanical microscope in combination to advanced optics. The combination of these techniques will allow us to study ion channels structural dynamcs and link it to their physiological function. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The SthK channel gene (UniProtKB G0GA88)was subcloned in the appropriate vector (pCGFP-BC) for overexpression in bacteria (BL21-DE3). Channel expression, purification and reconstitution in artificial bilayer was implemented successfully. The sample was characterized by means of force-spectroscopy (i.e. adsorbed to the AFM probe and unfolded from the membrane bilayer) and high-speed AFM imaging both in the presence of the endogenous ligand (cAMP) and its absence. This allowed us to define key interactions among the different protein domains and the overall mechanisms of gating (i.e. the opening of the permeation pathway). One paper, in collaboration with the ISAS-Italy (International School for Advanced Studies) is currently in revision and another is in preparation. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 研究課題/領域番号:20K22626, 研究期間(年度):2020-09-11 – 2022-03-31 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 出典：研究課題「Nanoscale probing and visualization of cyclic nucleotide-gated ion channels structural dynamics and function」課題番号20K22626 （KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20K22626）を加工して作成 |
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| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://kaken.nii.ac.jp/ja/search/?kw=80882240 | |||||
| 関連名称 | https://kaken.nii.ac.jp/ja/search/?kw=80882240 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20K22626 | |||||
| 関連名称 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20K22626 | |||||
