{"created":"2023-07-27T06:56:39.797755+00:00","id":53548,"links":{},"metadata":{"_buckets":{"deposit":"920045a2-ff71-4b12-bebb-a44dd86126bc"},"_deposit":{"created_by":18,"id":"53548","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"53548"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00053548","sets":["2812:2813:2826"]},"author_link":["91653","37"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-02-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2005 – 2007","bibliographic_titles":[{"bibliographic_title":"平成19(2007)年度 科学研究費補助金 基盤研究(B) 研究成果報告書概要"},{"bibliographic_title":"2007 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々は従来から遺伝子治療のベクターとして用いられてきたウイルスの毒性に着目し、ウイルス自らが生体内で増殖複製し、その感染毒性により細胞を死滅させるシステムを模索してきた。また我々はテロメレース触媒サブユニットであるhTERT(Human telomerase reverse transcriptase)遺伝子プロモーターを世界で初めてクローニングし、このプロモーターが癌細胞特異的に活性化されることを報告してきた。本研究ではhTERTプロモーターをアデノウイルスtype5の複製に必要な自己蛋白E1Aの遺伝子の上流に組み込んだ増殖型ウイルス(TRAD: telomerase-specific replication-competent adenovirus)を開発し、in vitroおよびin vivoで腫瘍特異的増殖を確認するとともに、その抗腫瘍効果を明らかにした。TRADの腫瘍への直接注入により明確な造腫瘍抑制効果が認められる一方で、他臓器への毒性は認められなかった。一方でTRADは注入部位より拡散し、血流に乗って遠隔病巣にも到達し得ることが明らかとなった。このことは転移病巣にも有効である可能性を示唆する。またTRADは抗癌剤耐性細胞にも有効であり、さらには既存の抗癌剤との併用で相乗作用を有することも報告してきた。現在このウイルスはOncolys社との共同開発により米国で臨床試験を行っている。","subitem_description_type":"Abstract"},{"subitem_description":"We previously cloned the promoter of human reverse transcriptase (hTERT) gene, a catalytic subunit of human telomerase, which was found to be active only in cancer cells with telomerase activity, while it was silent in most normal cells without telomerase activity. We have planned to insert this promoter into the upstream of the El gene of Adenovirus type 5 genome so that adenovirus El gene is efficiently expressed only in cancer cells. Eventually, this chimera virus can replicate only in cancer cells with high levels of expression of El gene, which is required for viral replication. We named this virus TRAD: telomerase-specific replication adenovirus. In the present project, we examined the in vitro and in vivo efficacy of TARD directly injected into primary site of tumors. We found that TARD was effective to reduce tumor burden without exhibiting any severe adverse effect, such as myelosurpression or liver dysfunction caused by viral toxicity Therefore, we propose the clinical use of this virus.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:17390449, 研究期間(年度):2005 – 2007","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「テロメレース特異的癌融解ウイルスによる婦人科腫瘍の遺伝子治療の開発」課題番号17390449\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-17390449/173904492007kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00059830","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=50272969"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=50272969","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17390449/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17390449/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-17390449/173904492007kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-17390449/173904492007kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-16"}],"displaytype":"detail","filename":"ME-PR-KYO-S-kaken 2010-2p.pdf","filesize":[{"value":"89.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KYO-S-kaken 2010-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/53548/files/ME-PR-KYO-S-kaken 2010-2p.pdf"},"version_id":"4c33103b-aded-4d72-8c7a-980b47d22676"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"テロメレース特異的癌融解ウイルスによる婦人科腫瘍の遺伝子治療の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"テロメレース特異的癌融解ウイルスによる婦人科腫瘍の遺伝子治療の開発"},{"subitem_title":"Establishment of novel cancer therapy using telomerase-specific replication competent adenovirus","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2826"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-16"},"publish_date":"2022-05-16","publish_status":"0","recid":"53548","relation_version_is_last":true,"title":["テロメレース特異的癌融解ウイルスによる婦人科腫瘍の遺伝子治療の開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:06:38.919266+00:00"}