{"created":"2023-07-27T06:56:41.880299+00:00","id":53594,"links":{},"metadata":{"_buckets":{"deposit":"9f0f320e-7ad4-425b-af07-a3e6e86e811c"},"_deposit":{"created_by":18,"id":"53594","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"53594"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00053594","sets":["2812:2813:2820"]},"author_link":["95228"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2012-04-01 – 2014-03-31","bibliographic_titles":[{"bibliographic_title":"平成25(2013)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2013 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kanda, Natsuko"}],"nameIdentifiers":[{"nameIdentifier":"95228","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40432166","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40432166"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"視索前野GABA作動性神経は睡眠調節に重要と考えられている。本研究は視索前野GABA作動性神経特異的にChannelrhodopsin2を発現させ、その投射先を示した。またDREADDシステムを用いて、視索前野GABA作動性神経特異的に神経活動を抑制または興奮させたときのマウスの睡眠を測定した。GABA作動性神経の興奮はNREM睡眠時間を延長し、抑制はREM睡眠の減少および明期のNREM睡眠の増加、さらに暗期の覚醒持続時間の減少を起こした。また、視索前野GABA作動性神経終末の興奮は、オレキシン作動性神経の発火頻度を減少させることが分かり、この抑制経路は睡眠調節経路の一つである可能性を示した。","subitem_description_type":"Abstract"},{"subitem_description":"GABAergic neurons in the preoptic area (POA) is thought to have a important role in sleep regulation. We applied DREADD system to POA GABAergic neurons to modulate activities of the neurons. Activation of POA GABAergic neurons increased total NREM sleep time. Inhibition of the neurons reduced REM sleep time and increased NREM sleep time during light period and reduced episode durations of wakefulness during dark period.\nAxonal projections of POA GABAergic neurons were observed in hypothalamic area where orexin neurons localized using Channelrhodopsin2 (ChR2)as an anterograde tracer. Moreover, light stimulation of axonal terminals of POA GABAergic neuron which expressed ChR2 inhibited firings of orexin neurons in slice preparation. These results suggest that the functional connection between POA GABAergic neurons and orexin  neurons might be an important pathway in sleep regulation.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:24700322, 研究期間(年度):2012-04-01 – 2014-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「視索前野GABA作動性神経の遺伝薬理学的および光操作による睡眠回路解析」研究成果報告書　課題番号24700322\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24700322/24700322seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"筑波大学 / 金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00059876","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40432166"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40432166","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700322/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700322/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24700322/24700322seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24700322/24700322seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"辻野, なつ子"}],"nameIdentifiers":[{"nameIdentifier":"95228","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40432166","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40432166"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-20"}],"displaytype":"detail","filename":"ME-PR-KANDA-N-kaken 2014-4p.pdf","filesize":[{"value":"90.7 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KANDA-N-kaken 2014-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/53594/files/ME-PR-KANDA-N-kaken 2014-4p.pdf"},"version_id":"153a3b6c-4024-4c3e-ae72-51eb13c003f9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"視索前野GABA作動性神経の遺伝薬理学的および光操作による睡眠回路解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"視索前野GABA作動性神経の遺伝薬理学的および光操作による睡眠回路解析"},{"subitem_title":"Research on the role of the POA GABAergic neuron in the sleep regulation.","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2820"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-20"},"publish_date":"2020-11-20","publish_status":"0","recid":"53594","relation_version_is_last":true,"title":["視索前野GABA作動性神経の遺伝薬理学的および光操作による睡眠回路解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:51:59.575660+00:00"}