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The Role of Lipophilic Quinones in the Electron Transport System of Escherichia coli
https://doi.org/10.24517/00060076
https://doi.org/10.24517/00060076b190ba66-5857-478a-8658-bf8abd98eed4
| 名前 / ファイル | ライセンス | アクション |
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SC-PR-ITAGAKI-E-55_432.pdf (972.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2020-12-03 | |||||||
| タイトル | ||||||||
| タイトル | The Role of Lipophilic Quinones in the Electron Transport System of Escherichia coli | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00060076 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Itagaki, Eiji
× Itagaki, Eiji |
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| 著者別表示 |
板垣, 英治
× 板垣, 英治
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| 書誌情報 |
The Journal of Biochemistry 巻 55, 号 4, p. 432-445, 発行日 1964 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0021-924X | |||||||
| NCID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00694073 | |||||||
| 出版者 | ||||||||
| 出版者 | The Japanese Biochemical Society | |||||||
| 出版者（別名） | ||||||||
| 出版者 | 日本生化学会 | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | A pale-brown powder preparation of the particulate fraction of E. coli cells grown with nitrate respiration was obtained with cold acetone treatment. This preparation, was similar to the solubilized and partially purified enzyme preparation, had high activities of for-mate dehydrogenase and nitrate reductase and contained cytochrome b1, but no activity of formate-nitrate reductase system. By addition of lipids prepared from this organism with aid of a detergent, PADA, the activity of formate-nitrate reductase system was completely restored. The restoration efficiencies of enzyme activity by UQ8 and K2 which were quinones found in this organism, were 2.6 to 1 per mole. The experiments with the UQ8, K2 or both specifically deprived, and ultraviolet irradiated lipid preparations showed that UQ8, rather than K2, acted more effectively as an electron carrier. UQ8 was anaerobically reduced slowly by the enzyme preparation wit formate, and UQ8H2 was rapidly oxidized by nitrate with simultaneous production of stoichiometric amounts of nitrite. From these results, it is concluded taht UQ8 acts principally as hydrogen acceptor for formate dehydrogenase of E. coli. The author is very grateful to Professer R. Sato of this laboratory for his helpful advice, to Mr. T. Fujita of his continous discussion to this work, to Dr. S. Ikeda of the Faculty of Science, Osaka University for his helpful advice to the synthesis of PADA, to Dr. K. Tanaka of Takeda Pharmaceutical Industries Ltd., Osaka and Dr. K. Uehara of the Pharmac-eutical Faculty of Osaka University for materials, and to Dr. K. Kusai of Nagase Sangyo Co. Ltd. for large culture of the organism. |
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| 権利 | ||||||||
| 権利情報 | Copyright © The Japanese Biochemical Society 日本生化学会 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | http://www.jbsoc.or.jp/ | |||||||
| 関連名称 | http://www.jbsoc.or.jp/ | |||||||
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| 識別子タイプ | URI | |||||||
| 関連識別子 | https://academic.oup.com/jb | |||||||
| 関連名称 | https://academic.oup.com/jb | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.jstage.jst.go.jp/browse/biochemistry1922/-char/ja | |||||||
| 関連名称 | https://www.jstage.jst.go.jp/browse/biochemistry1922/-char/ja | |||||||
