{"created":"2023-07-27T06:57:20.750347+00:00","id":54508,"links":{},"metadata":{"_buckets":{"deposit":"d0fc60c6-f3a2-4274-9cb2-4113357546d3"},"_deposit":{"created_by":18,"id":"54508","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"54508"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00054508","sets":["2812:2813:2834"]},"author_link":["95935"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1998 – 1999","bibliographic_titles":[{"bibliographic_title":"平成11(1999)年度 科学研究費補助金 奨励研究(A) 研究概要"},{"bibliographic_title":"1999 Research Project Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yokoyama, Ken"}],"nameIdentifiers":[{"nameIdentifier":"95935","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70271377","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70271377"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"吉田らの方法に準拠して、V1-ATPaseの回転の観察を行うために、遺伝子操作によりローターサブユニットである、ガンマsubunitにシステイン残基を導入したmutantV1を合計10種類作成した(ガンマサブユニット2,32,58,90,95,100,116,120,144,163のセリン、 もしくはアラニンをシステインに置換)。\n蛍光化されたアクチン線維をローターサブユニットに結合させるためには、ガンマのシステイン残基をNEM-ビオチン化する必要がある。作成したmV1うち明確にビオチン化されるのは100番目のものだけであった。現在、このmutantV1を用いて回転の観察をおこなっている。いまのところ、明確な回転を観察できていない。\nこの実験と平行して、ガンマsubnitの回転と共役したc-subunit oligomerの回転の観察も試みている。そのためにVoV1 operonのクローニングを行い、全塩基配列の決定と、構成subunitの決定に成功した(JBC274,inpress,on May)。現在、VoV1の発現系の構築を試みている。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:10780404, 研究期間(年度):1998 – 1999","subitem_description_type":"Other"},{"subitem_description":"出典：「V型ATP合成酵素の回転の直接観察」研究成果報告書　課題番号10780404\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10780404/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00060784","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=70271377"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=70271377","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10780404/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10780404/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"横山, 謙"}],"nameIdentifiers":[{"nameIdentifier":"95935","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70271377","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70271377"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-08"}],"displaytype":"detail","filename":"PH-PR-YOKOYAMA-K-kaken 2016-2p.pdf","filesize":[{"value":"121.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-YOKOYAMA-K-kaken 2016-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/54508/files/PH-PR-YOKOYAMA-K-kaken 2016-2p.pdf"},"version_id":"c795ff93-b13c-4d46-a862-7713d49e501b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"V型ATP合成酵素の回転の直接観察","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"V型ATP合成酵素の回転の直接観察"}]},"item_type_id":"9","owner":"18","path":["2834"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-08"},"publish_date":"2021-10-08","publish_status":"0","recid":"54508","relation_version_is_last":true,"title":["V型ATP合成酵素の回転の直接観察"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T06:33:15.666335+00:00"}