{"created":"2023-07-27T06:57:24.389676+00:00","id":54592,"links":{},"metadata":{"_buckets":{"deposit":"b9fc5bc3-c634-4dcb-bbd1-77571c30fd03"},"_deposit":{"created_by":18,"id":"54592","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"54592"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00054592","sets":["2812:2813:2835"]},"author_link":["26429","60141"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1997 – 1998","bibliographic_titles":[{"bibliographic_title":"平成10(1998)年度 科学研究費補助金 奨励研究(A) 研究概要"},{"bibliographic_title":"1998 Research Project Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本年度は昨年度の研究を踏まえ,LC/MSにおける誘導体化の有用性をさらに明らかにすると共に,生体試料中微量ステロイド分析に応用した.\n1. ビタミンD骨格と選択的に反応するCookson型試薬(4-phenyl-1,2,4-triazoline-3,5-dione等)を用いてビタミンD脂肪酸エステルを誘導体化し,LC/APCI-MSに付したところ,正負両イオンモードで分子量関連イオン([M+H]^+又は[M-H]^-)のみならず特徴的なフラグメントイオンが生成し,抱合位置に関する情報も得られた.これは生体試料中ビタミンD代謝物の同定上有用な知見となり得るものであった.\n2. 脳内ステロイドホルモンの一種であるプレグネノロン 3-サルフェート(PS)に着目し,まず標品PSをO-methylhydroxylamine,O-pentafluorobenzylhydroxylamine又は4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole(DBD-H)で誘導体化後,負イオン検出LC-ESI-MSに付し,その諸性質を精査した.その結果,いずれも[M-H]^-が基準イオンとして観察される上に,本イオンを用いる選択イオン検出において,誘導体化前に比し約10倍の高感度な応答を示し,誘導体化の有用性が明らかとなった.また,誘導体化率,後処理の簡便性,実試料に適用した際のクロマトグラフ的挙動を考慮した結果,上記の誘導体化の中でDBD化が最も優れていた.\n3. 上記2の誘導体化法及び重水素化内標準物質[^2H_4]PSを用い,LC/MS(/MS)によるラット脳内PS定量法を開発し,実試料へ適用した.その結果,従来法(RIA等)による文献値(21±5 ng/g tissue)に比し,はるかに低値を示すなど,興味ある知見が得られた.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:09771943, 研究期間(年度):1997 – 1998","subitem_description_type":"Other"},{"subitem_description":"出典：「LC/MS/MSの生体内超微量ステロイド分析への応用」研究成果報告書　課題番号09771943\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-09771943/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00060868","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=70242526"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=70242526","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-09771943/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-09771943/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-12"}],"displaytype":"detail","filename":"PH-PR-MITAMURA-K-kaken 2016-2p.pdf","filesize":[{"value":"152.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-MITAMURA-K-kaken 2016-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/54592/files/PH-PR-MITAMURA-K-kaken 2016-2p.pdf"},"version_id":"90269a51-d681-4652-90c1-7ddb4a5e6c15"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"LC/MS/MSの生体内超微量ステロイド分析への応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"LC/MS/MSの生体内超微量ステロイド分析への応用"}]},"item_type_id":"9","owner":"18","path":["2835"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-12"},"publish_date":"2021-11-12","publish_status":"0","recid":"54592","relation_version_is_last":true,"title":["LC/MS/MSの生体内超微量ステロイド分析への応用"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:21:20.733736+00:00"}