{"created":"2023-07-27T06:57:39.989505+00:00","id":54941,"links":{},"metadata":{"_buckets":{"deposit":"343dc651-ac46-434f-b26f-7b5690f51db7"},"_deposit":{"created_by":18,"id":"54941","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"54941"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00054941","sets":["2812:2813:2831"]},"author_link":["1818"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"1p.","bibliographicVolumeNumber":"2001 – 2002","bibliographic_titles":[{"bibliographic_title":"平成14(2002)年度 科学研究費補助金 若手研究(B) 研究概要"},{"bibliographic_title":"2002 Research Rroject Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kinoshita, Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"1818","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20311681","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20311681"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"昨年度単離したBid結合因子(clone-2)の機能解析を進めた。1,clone-2遺伝子の発現分布をNorthern Blotで解析した。正常組織(CLONTECH社のMultiple Tissue Northern Blotを利用した)ではBrain、Liverで強いmRNAの発現を検出した。培養細胞(Cos7,Hela, HEK293,HepG2,Jurkat K562,THP1)ではHepG2細胞でやや強く、Cos7,HEK293で弱いmRNA発現を検出した。RT-PCR解析では全てのcell lineで発現が確認された。2,clone-2の安定発現細胞株の樹立を試みた。Jurkat細胞にのcDNAを組み込んだpIRES2-EGFP(CLONTECH社)ベクターを導入し、G418耐性、GFP発現を指標にclone-2の安定発現クローンを4種類得た。4クローン中2クローンがレコンビナントFasリガンドで誘導されるアポトーシスに耐性を示した。3,clone-2の細胞内局在を調べた。clone-2に対するポリクローナル抗体を作製し、HepG2細胞を免疫染色したところ、ミトコンドリアに強い染色像が見られた。HepG2細胞を分画し、western blot解析したところ、ミトコンドリアを含むheavy membrane画分にclone-2のカルボキシ末端側とみられる14kDaのバンドを検出した。clone-2の全長に相当する蛋白のバンドは確認できなかった。4,アンチセンスRNA発現ベクター導入による内在性clone-2の発現阻害を試みたが、14kDaのバンドの消失は認められなかった。\n以上の結果より、過剰発現系においてはclone-2がBidで誘導されるアポトーシスに対して抑制的に働く可能性が示唆された。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:13770106, 研究期間(年度):2001-2002","subitem_description_type":"Other"},{"subitem_description":"出典：「アポトーシス促進因子Bidの制御機構」研究成果報告書　課題番号13770106\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（ https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-13770106/ ）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん進展制御研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00061217","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=20311681"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=20311681","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-13770106/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-13770106/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木下, 健"}],"nameIdentifiers":[{"nameIdentifier":"1818","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20311681","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20311681"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-04"}],"displaytype":"detail","filename":"CA-PR-KINOSHITA-T-kaken 2016-1p.pdf","filesize":[{"value":"111.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-KINOSHITA-T-kaken 2016-1p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/54941/files/CA-PR-KINOSHITA-T-kaken 2016-1p.pdf"},"version_id":"537ddcc7-2c26-434b-b3f1-aa6ac8b53a64"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"アポトーシス促進因子Bidの制御機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アポトーシス促進因子Bidの制御機構"}]},"item_type_id":"9","owner":"18","path":["2831"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-03-04"},"publish_date":"2021-03-04","publish_status":"0","recid":"54941","relation_version_is_last":true,"title":["アポトーシス促進因子Bidの制御機構"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T06:26:40.506509+00:00"}