{"created":"2023-07-27T06:57:59.354412+00:00","id":55523,"links":{},"metadata":{"_buckets":{"deposit":"86f8c69e-539a-4c48-b97c-17fc2b3411c0"},"_deposit":{"created_by":18,"id":"55523","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"55523"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00055523","sets":["2812:2813:2826"]},"author_link":["88087","88086"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06-17","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"1p.","bibliographicVolumeNumber":"2006 – 2007","bibliographic_titles":[{"bibliographic_title":"平成19(2007)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"2007 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ADAMTS-1は、ADAMTSファミリーの最初のメンバーとして我々が同定した遺伝子である。我々はこれまでにADAMTS-1遺伝子欠損マウスの解析から、ADAMTS-1が腎盂尿管移行部組織の構築と機能、排卵過程、卵胞生育過程と卵胞構造の維持等に関わることを明らかにしてきた。皮膚損傷治癒過程は、炎症期、増殖期、成熟期の3つの段階からなり、炎症反応を含む連続的な組織の修復過程である。我々はADAMTS1が炎症時にその発現が誘導される遺伝子であることを見い出していたことから、今回、ADAMTS-1遺伝子欠損マウスを用いて、ADAMTS-1が皮膚損傷治癒過程に関与しているかどうか検討を行った。実験としては8-12週令のADAMTS-1遣伝子欠損マウスおよびコントロールマウスの背部の皮膚に、生検トレパンを用いて直径4mmの打ち抜き損傷を作製し、経時的に損傷部位の面積を測定して、治癒過程の進行度に違いがあるか調べた。その結果、ADAMTS-1遺伝子欠損マウスでは、損傷作製後3日目から、コントロールマウスと比較して治癒の遅れが認められはじめた。またその後、損傷作製後6日目から10日目にかけても、ADAMTS-1遺伝子欠損マウスでの損傷部位の面積は、コントロールマウスと比較して大きいままで、損傷部位の塞がり方に明らかな遅れが観察された。これらの結果から、ADAMTS-1が皮膚損傷治癒過程において重要な役割を果たしていることが示唆された。我々がこの結果を見い出した後、他のグループより、ADAMTS-1が皮膚損傷過程の血管新生の制御に関わっていることが報告されたが、今後、ADAMTS-1遺伝子欠損マウスにおける皮膚損傷治癒過程の遅延のメカニズムについては、さらに詳しい解析が必要である。","subitem_description_type":"Abstract"},{"subitem_description":"ADAMTS-1 is a metalloproteinase associated with extracellular matrix that has proteoglycandegrading activity and anti-angiogenic activity. By analyzing ADAMTS-1 gene knockout mice, we have shown that ADAMTS-1 is a multifunctional metalloproteinase that is essential for the structure and function of the ureteropelvic junction as well as ovulation, follicular development, and maintenance of the follicle structure in the ovary. Skin wound healing is a multiple-step process consisting of an inflammation phase, a proliferation phase, and a maturation phase. Since ADAMTS-1 is a gene whose expression is upregulated in the inflammatory condition, in the present study we evaluated whether ADAMTS-1 is involved in the skin wound healing process. Excisonal full-thickness skin wounds were made on the dorsal skin of ADAMTS-1 null mice with a sterile disposable biopsy punch with a diameter 4mm, and changes in wound areas were monitored as an index of wound repair. Three days after injury, wound closure in ADAMTS-1 null mice was delayed compared with that in control mice. Thereafter, the wound area of ADAMTS-1 null mice remained larger than those of control mice until ten days after injury. These observations demonstrated that ADAMTS-1 plays an important role in the skin wound healing process. After we found this phenomenon, another research group reported that ADAMTS-1 regulates the angiogenic response during tissue repair, but further investigation will be necessary to understand the detailed mechanism of delayed wound healing in ADAMTS-1 null mice.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18590365, 研究期間(年度):2006 – 2007","subitem_description_type":"Other"},{"subitem_description":"出典：「ノックアウトマウスを用いたADAMTS-1の皮膚損傷治癒過程における役割の解析」研究成果報告書　課題番号18590365\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18590365/185903652007kenkyu_seika_hokoku_gaiyo/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん進展制御研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00061798","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=40242565"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=40242565","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18590365/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18590365/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18590365/185903652007kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18590365/185903652007kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-17"}],"displaytype":"detail","filename":"CA-PR-KUNO-K-kaken 2011-1p.pdf","filesize":[{"value":"149.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-KUNO-K-kaken 2011-1p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/55523/files/CA-PR-KUNO-K-kaken 2011-1p.pdf"},"version_id":"bcb9412a-e621-4088-9616-a36fcdf09695"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ノックアウトマウスを用いたADAMTS-1の皮膚損傷治癒過程における役割の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ノックアウトマウスを用いたADAMTS-1の皮膚損傷治癒過程における役割の解析"},{"subitem_title":"Study of function of ADAMTS-1 in skin wound healing","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2826"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-05-17"},"publish_date":"2021-05-17","publish_status":"0","recid":"55523","relation_version_is_last":true,"title":["ノックアウトマウスを用いたADAMTS-1の皮膚損傷治癒過程における役割の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:22:41.108035+00:00"}