{"created":"2023-07-27T06:57:59.624884+00:00","id":55559,"links":{},"metadata":{"_buckets":{"deposit":"958b48a1-b1ef-4cc9-a6fe-f74ab6c6a5ac"},"_deposit":{"created_by":18,"id":"55559","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"55559"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00055559","sets":["2812:2813:2826"]},"author_link":["80517","2640"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-02-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2006 – 2007","bibliographic_titles":[{"bibliographic_title":"平成19(2007)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"2007 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"再生医工学またはティッシュ・エンジニアリングと呼ばれる技術により、失われた組織を患者自身から採取した細胞を担体(scaffold)内で培養することで再生する試みが注目されている。この技術は、従来の人工生体材料が持つ生体適合性や力学的適合性における諸問題を克服できる可能性を有している。骨組織においても同様の試みが行われているものの、未だ培養下において骨組織と同等の石灰化度を有する再生骨を得るには至っていない。そのため、担体の初期剛性もしくは靭性が低く、荷重負荷のかかる重要箇所への移植には適さない。そこで本研究課題では、力学刺激を与えることで再生骨の石灰化を促進し、同時に石灰化度情報に基づき力学刺激間隔を最適化することで最大石灰化を得ることのできる'力学刺激適応型再生骨培養システム'を構築することを目的とした。\n当該研究期間内では主に、同システムの構築に必要な要素技術の開発を行った。平成18年度においては、近赤外光を用いた再生骨の石灰化を非破壊的にモニタリングするシステムを開発し、その有効性をラット骨芽細胞を三次元培養した再生骨を用いて確認した。平成19年度では、力学刺激間隔フィードバック制御開始時の刺激の初期条件を決定する方法の検討を行った。すなわち、培養初期の再生骨が確実に反応する力学刺激条件を早期に決定するために刺激直後に生じる細胞内Ca2+の変化に着目した。そして、前述の石灰化モニタリングシステムを改良した装置を用いることで再生骨に力学刺激を与えた際の細胞内Ca2+変動を観察することに成功した。以上の成果は、力学刺激適応型再生骨培養システムの構築を大きく前進させるものであると言える。","subitem_description_type":"Abstract"},{"subitem_description":"Stem cells have a potential to differentiate to specific organ cells and could be utilized to regenerate a lost organ with an appropriate scaffold in culture. This technology called tissue engineering or regenerative medicine is a promising strategy with a high biocompatibility avoiding the ethical problems in cadaveric donor transplantation and living donor transplantation Tissue-engineered bone composed of osteoblasts, a scaffold, and simulative hums was studied and developed in the past years, however it is still difficult to obtain sufficient calcification in vitro, comparing with natural bones. With this low mechano-compatibility, in particular a shortage of initial stiffness and toughness, tissue-engineered bone is not applicable to implantation to loaded bone sites. The purpose of this project is to development of a feed-back controlled culture system to maximize the calcification of tissue-engineered bone promoted by mechanical stimulation in fain of strain-induced fluid flow. This culture system is expected to provide better mechano-compatibility to an engineered bone before implantation. In this two-year project (2006-2007), two elemental technologies for the system were developed. In 2006, an optical device for non-destructive monitoring of in vitro calcification was developed using near-infrared light Using this device, the calcification of tissue-engineered bone composed of a type I collagen sponge and rat-primary cultured osteoblasts was monitored for 42 days in culture. In 3307, in the purpose of determination of initial condition of mechanical stimulation, a compact optical device was developed to observe concentration changes in intracellular calcium in osteoblasts populated in an engineered bone just after mechanical stimulation. These achievements should contribute to completion of the feed-hack controlled culture system for tissue-engineered bone.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18500343, 研究期間(年度):2006 – 2007","subitem_description_type":"Other"},{"subitem_description":"出典：「ひずみ誘導型液体流動を用いた力学刺激適応型再生骨培養システムの開発」研究成果報告書　課題番号1850034\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18500343/185003432007kenkyu_seika_hokoku_gaiyo/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学環日本海域環境研究センター","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00061834","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=20262602"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=20262602","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18500343/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18500343/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18500343/185003432007kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-18500343/185003432007kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-17"}],"displaytype":"detail","filename":"OC-PR-TANAKA-S-kaken 2010-2p.pdf","filesize":[{"value":"187.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"OC-PR-TANAKA-S-kaken 2010-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/55559/files/OC-PR-TANAKA-S-kaken 2010-2p.pdf"},"version_id":"9ec75bee-f9a1-4bfc-84bc-e6464a5c7778"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ひずみ誘導型液体流動を用いた力学刺激適応型再生骨培養システムの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ひずみ誘導型液体流動を用いた力学刺激適応型再生骨培養システムの開発"},{"subitem_title":"Development of a feed-back controlled culture system to maximize calcification of tissue-engineered hone promoted by strain-induced fluid flow","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2826"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-05-17"},"publish_date":"2021-05-17","publish_status":"0","recid":"55559","relation_version_is_last":true,"title":["ひずみ誘導型液体流動を用いた力学刺激適応型再生骨培養システムの開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:22:33.446910+00:00"}