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Post-mortem Plasma Cell-Free DNA Sequencing: Proof-of-Concept Study for the “Liquid Autopsy”
https://doi.org/10.24517/00062702
https://doi.org/10.24517/0006270246a2a6f1-c8e9-43d7-82e2-e3fe9bef8ee2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME-PR-MAEDA-D-2120.pdf (3.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2021-06-28 | |||||||
| タイトル | ||||||||
| タイトル | Post-mortem Plasma Cell-Free DNA Sequencing: Proof-of-Concept Study for the “Liquid Autopsy” | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00062702 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Takai, Erina
× Takai, Erina× Maeda, Daichi× Li, Zhuo× Kudo-Asabe, Yukitsugu× Totoki, Yasushi× Nakamura, Hiromi× Nakamura, Akiko× Rumi Nakamura× Kirikawa, Misato× Ito, Yukinobu× Yoshida, Makoto× Inoue, Takamitsu× Habuchi, Tomonori× Ikoma, Shohei× Katoh, Hiroto× Kato, Mamoru× Shibata, Tatsuhiro× Ishikawa, Shumpei× Yachida, Shinichi× Goto, Akiteru |
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| 著者別表示 |
前田, 大地
× 前田, 大地
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学医薬保健研究域医学系 | |||||||
| 書誌情報 |
Scientific Reports 巻 10, 号 1, p. 2120, 発行日 2020-02-07 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 2045-2322 | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1038/s41598-020-59193-y | |||||||
| 出版者 | ||||||||
| 出版者 | Nature Research | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Recent genomic studies on cancer tissues obtained during rapid autopsy have provided insights into the clonal evolution and heterogeneity of cancer. However, post-mortem blood has not been subjected to genetic analyses in relation to cancer. We first confirmed that substantial quantities of cell-free DNA were present in the post-mortem plasma of 12 autopsy cases. Then, we focused on a pilot case of prostate cancer with multiple metastases for genetic analyses. Whole-exome sequencing of post-mortem plasma-derived cell-free DNA and eight frozen metastatic cancer tissues collected during rapid autopsy was performed, and compared their mutational statuses. The post-mortem plasma cell-free DNA was successfully sequenced and 344 mutations were identified. Of these, 160 were detected in at least one of the metastases. Further, 99% of the mutations shared by all metastases were present in the plasma. Sanger sequencing of 30 additional formalin-fixed metastases enabled us to map the clones harboring mutations initially detected only in the plasma. In conclusion, post-mortem blood, which is usually disposed of during conventional autopsies, can provide valuable data if sequenced in detail, especially regarding cancer heterogeneity. Furthermore, post-mortem plasma cell-free DNA sequencing (liquid autopsy) can be a novel platform for cancer research and a tool for genomic pathology. © 2020, The Author(s). | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright © Nature Research | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | http://www.nature.com/srep/index.html | |||||||
| 関連名称 | http://www.nature.com/srep/index.html | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.nature.com/articles/s41598-020-59193-y | |||||||
| 関連名称 | https://www.nature.com/articles/s41598-020-59193-y | |||||||
