{"created":"2023-07-27T06:58:26.348720+00:00","id":56821,"links":{},"metadata":{"_buckets":{"deposit":"e87d16a8-b113-4356-9fff-244261595819"},"_deposit":{"created_by":18,"id":"56821","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"56821"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00056821","sets":["2812:2813:2828"]},"author_link":["77112","99427"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-12-12","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2004 – 2005","bibliographic_titles":[{"bibliographic_title":"平成17(2005)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"2005 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究ではDNAメチル化プロファイリングを肺癌の予後判定、個別化治療へ応用することを目的に、過去に癌の診断・治療が行われた症例の臨床データをretrospectiveに解析し、同時に各症例の癌組織より抽出したDNAのメチル化パターンを解析した。\n臨床的な組織保存法として一般的な、ホルマリン固定パラフィン包埋ブロックからDNAを抽出し、DNAメチル化を解析する手法を確立した後、肺癌350例の腫瘍組織および近接正常組織よりDNAを抽出した。抽出したDNAをbisulfite処理し、real-time methylation specific PCR法(MethyLight法)によりp16, hMLH1, APC, MGMT, DAPK, MYOD1, TIMP3のプロモーター領域におけるDNAメチル化を定量した。腫瘍組織(T)と正常組織(N)におけるそれぞれの遺伝子のhypermethylation(HM)頻度は、p16(T),14.1%;p16(N),1.8%;hMLH1(T),0.7%;hMLH1(N),0%;APC(T),24.8%;APC(N),8.8%;MGMT(T),67.2%;MGMT(N),87.5;DAPK(T),1.6%;DAPK(N),3.6%;MYOD1(T),42.2%;MYOD1(N),34.4%;TIMP3(T),3.1%;TIMP3(N),3.6%であった。腫瘍組織でのメチル化と臨床病理学的因子の関連を検討すると、p16のメチル化は有意に男性および扁平上皮癌に多く、一方APCのメチル化は腺癌で有意に多かった。240例を対象に予後との関連を検討すると、p16, MYOD1のメチル化は予後不良因子であった。以上の結果から、各遺伝子のDNAメチル化プロファイルを総合的に判定し、肺癌の個性診断および個別化治療に応用可能であることが示された。","subitem_description_type":"Abstract"},{"subitem_description":"Despite recent advances in cancer therapy, lung cancer remains one of the major causes of cancer death worldwide. Therefore, precise methods for prediction of prognosis are required for optimal selection from among the available modalities of lung cancer therapy. In this study, DNA methylation profile of tumor and normal tissue in lung cancer was analyzed to make use of the profile for personalized medicine in the treatment of this disease.\nThe method of real-time methylation specific PCR (MethyLight) from formalin-fixed paraffin-embedded tissue was established. DNA was isolated from 350 matched tumor and adjacent normal tissue, followed by MethyLight assay on p16, hMLH1, APC, MGMT, DAPK, MYOD1, TIMP3. Frequencies of the hypermethylation in tumor (T) and normal tissue (N) were as follows : p16(T), 14.1% ; p16(N), 1.8% ; hMLH1(T), 0.7% ; hMLH1(N), 0% ; APC(T), 24.8% ; APC(N), 8.8% ; MGMT(T), 67.2% ; MGMT(N), 87.5 ; DAPK(T), 1.6% ; DAPK(N), 3.6% ; MYOD1(T), 42.2% ; MYOD1(N), 34.4% ; TIMP3(T), 3.1% ; TIMP3(N), 3.6%. Hypermethylation of p16 was more frequent in male and squamous cell carcinoma. Contrary, APC hypermethylation was more frequent in adenocarcinoma. In survival analysis with 240 patients, hypermethylation of p16 and MYOD1 were significant poor prognostic factor.\nThese results suggest that the DNA methylation profile in lung cancer is useful for personalized medicine in the treatment of lung cancer patients.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16591386, 研究期間(年度):2004 – 2005","subitem_description_type":"Other"},{"subitem_description":"出典：「DNAメチル化プロファイリングによる肺癌の個性診断」研究成果報告書　課題番号16591386\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16591386/165913862005kenkyu_seika_hokoku_gaiyo/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学系研究科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00063095","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://nrid.nii.ac.jp/ja/search/?kw=00293358"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/search/?kw=00293358","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16591386/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16591386/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16591386/165913862005kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16591386/165913862005kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-08"}],"displaytype":"detail","filename":"ME-PR-KAWAKAMI-K-kaken 2007-2p.pdf","filesize":[{"value":"85.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KAWAKAMI-K-kaken 2007-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/56821/files/ME-PR-KAWAKAMI-K-kaken 2007-2p.pdf"},"version_id":"71502de9-8f01-42d2-a1cc-51253a71d960"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"DNAメチル化プロファイリングによる肺癌の個性診断","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNAメチル化プロファイリングによる肺癌の個性診断"},{"subitem_title":"Molecular characterization of lung cancer by DNA methylation profile","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2828"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-08"},"publish_date":"2021-11-08","publish_status":"0","recid":"56821","relation_version_is_last":true,"title":["DNAメチル化プロファイリングによる肺癌の個性診断"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:25:37.944471+00:00"}