{"created":"2023-07-27T06:58:33.992796+00:00","id":57180,"links":{},"metadata":{"_buckets":{"deposit":"fd9ee5be-80f7-4280-a16f-ebc5beb3aa38"},"_deposit":{"created_by":18,"id":"57180","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"57180"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00057180","sets":["2812:2813:2829"]},"author_link":["157"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-07-10","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2002 – 2004","bibliographic_titles":[{"bibliographic_title":"平成16(2004)年度 科学研究費補助金 基盤研究(B) 研究成果報告書概要"},{"bibliographic_title":"2004 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究課題ではin vitroおよびin vivoでの各浸潤モデルにおける浸潤増殖像の検討およびin vivoでは浸潤モデルにおける浸潤像と転移との関係についてそれぞれ検討した。その結果、コラーゲンゲルを用いたin vitro浸潤モデルにおいて各癌細胞の浸潤能に応じた浸潤像を再現することに成功し、浸潤様式が高度になるにつれゲル中へのび漫性の浸潤を認めた。また、浸潤様式4D型の癌細胞ではコラーゲンゲル中に線維芽細胞を入れない状態でも、同様にび漫性の浸潤を認めた。これらの細胞の運動能を検討した結果、浸潤能の高い細胞で浸潤時に自己分泌型の運動促進因子(autocrine motility factor ; AMF)の高い発現を観察し、高浸潤細胞では線維芽細胞の産生するHGF等に頼らず自ら産生する運動因子で浸潤する能力があることが証明された。基質分解能では各種のマトリックスメタロプロテナーゼ(MMP)を検討したところ浸潤能の高い細胞でMMP-2,MMP-9,MT1-MMPの過剰発現を認めた。接着能では細胞間接着分子であるカドヘリンおよびデスモグレインについて検索したところ、浸潤能の低い細胞の浸潤像では細胞間接着が強固に観察されたが、浸潤能の高い細胞の浸潤像では両者の接着分子はいずれも高度に消失していた。癌細胞周囲の間質反応を検索した結果では浸潤様式4D型において周囲に強い結合織造成が認められた。以上より口腔扁平上皮癌の浸潤像の形成には運動能、基質分解能、接着能が密接に関係していることが示唆された。また、最も高度な浸潤様式4D型では顕著な間質反応を伴いながら浸潤して行く様相が観察された。","subitem_description_type":"Abstract"},{"subitem_description":"Since 1978, we have been studying the mode of cancer invasion as the important prognostic factor in patients with oral squamous cell cancer. Grade 4 of mode of invasion proposed by Jakobsson was subclassified into grades 4C (Cord-like type) and 4D (Diffuse type). The purpose of this study is to immunohistochemically and experimentally elucidate this modified grading system for the mode of invasion. Materials and methods used are human cancer materials, DMBA induced tongue cancer and in vitro and in vivo invasion model by using 3 cell lines ; OCS-20 (Gr.3), OSC-19 (Gr.4C) and HOC-313 (Gr.4D). In immunostained human cancer materials, the higher the grade of the mode of invasion was, the less continuous the basement membrane was, and the less intercellular adhesion was. Majority of diffuse invasive cancers(Gr.4C and 4D) co-expressed membrane type-matrix metalloproteinase and matrix metalloprotenase-2. In cell motility assay, HOC-313 showed the greatest motility (autocrine motility factor ; AMF). Cell lines cultured on 3T3 fibroblast-embedded collagen gels showed similar invasion pattern to their origin. Among them, only HOC-313 showed invasion even if fibroblast was not embedded. In DMBA induced tongue cancer, the mode of invasion were observed from Gr.1 to Gr.4C. OSC-20 and OSC-19 implanted orthotopically in the tongue of nude mice grew and metastasized to lymph nodes. Their histologic appearance was similar to that of the origin. HOC-313 grew temporarily for several months by support of matrigel. In recent study, cell selected TSU (Gr.4D) produced lethal tumorgenicity in SCID mouse with similar histology to the origin. This grading system was also proven immunohistochemically and experimentally justifiable.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:14370666, 研究期間(年度):2002 – 2004","subitem_description_type":"Other"},{"subitem_description":"出典：「口腔扁平上皮癌の浸潤増殖動態に関する実験的研究-特に浸潤様式からみた浸潤機序の解析を中心に-」研究成果報告書　課題番号14370666\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-14370666/143706662004kenkyu_seika_hokoku_gaiyo/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学系研究科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00063450","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=00092445"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=00092445","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-14370666/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-14370666/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-14370666/143706662004kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-14370666/143706662004kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-10"}],"displaytype":"detail","filename":"ME-PR-YAMAMOTO-E-kaken 2006-2p.pdf","filesize":[{"value":"88.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-YAMAMOTO-E-kaken 2006-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/57180/files/ME-PR-YAMAMOTO-E-kaken 2006-2p.pdf"},"version_id":"b447e9b6-0bfa-40fa-b283-09b4c94e0baf"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"口腔扁平上皮癌の浸潤増殖動態に関する実験的研究: 特に浸潤様式からみた浸潤機序の解析を中心に","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"口腔扁平上皮癌の浸潤増殖動態に関する実験的研究: 特に浸潤様式からみた浸潤機序の解析を中心に"},{"subitem_title":"Experimental studies of invasion and metastasis activity of oral squamous cell carcinoma","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2829"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-07-10"},"publish_date":"2021-07-10","publish_status":"0","recid":"57180","relation_version_is_last":true,"title":["口腔扁平上皮癌の浸潤増殖動態に関する実験的研究: 特に浸潤様式からみた浸潤機序の解析を中心に"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:26:32.808829+00:00"}