{"created":"2023-07-27T06:58:35.162218+00:00","id":57249,"links":{},"metadata":{"_buckets":{"deposit":"c06cb868-6c53-4921-be81-1f7af77bb242"},"_deposit":{"created_by":18,"id":"57249","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"57249"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00057249","sets":["2812:2813:2831"]},"author_link":["2077"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-04-13","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2000 – 2002","bibliographic_titles":[{"bibliographic_title":"平成14(2002)年度 科学研究費補助金 基盤研究(B) 研究成果報告書概要"},{"bibliographic_title":"2002 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(1)hTERT・プロモーターの下流にアポトーシス誘導遺伝子の発現ベクターを組み込んだベクター(hTERT/caspase-8)を作製し、婦人科癌株化細胞に感染させるとがん細胞にアポトーシスが生じる。しかし、hTERT/caspase-8ではアポトーシスは癌細胞にのみ生じるが正常細胞には生じない。hTERT/caspase-8で治療すると癌細胞のみに効果が発現し、遺伝子治療の副作用軽減をはかれることを示した。\n(2)更に簡便性と安全性の高いBAVS(binary adenovirus system)を開発した。アポトーシス誘導遺伝子であるBaxはAd/GT-Bax発現ベクターを使うとGV-16により強力に活性化される。このことから、GV-16がhTERTプロモーターで癌細胞にのみ発現するベクターを使った一次システム(Ad/hTERT-GV16)に加えてGV-16で活性化されるAd/GT-Baxベクターを同時感染させる。この二段構えのシステムはより強力に正常細胞には障害なく癌細胞のみにアポトーシスを誘導した。\n(3)生体免疫能の増強策としてケモカインの一つである単球走化因子(MCP-1)の発現を増強させる系を開発した。癌細胞へのMCP-1遺伝子導入により細胞障害性T細胞が誘導されると同時に樹状細胞の集積が認められ、腫瘍の増殖が抑制された。さらに、シスプラチンを微量併用投与すると相乗効果が得られることを示した。","subitem_description_type":"Abstract"},{"subitem_description":"Telomerase activation has been observed in almost 90% of human cancers but not normal tissues of somatic origin. The recent research on the transcriptional regulation of hTERT promoter identified several factors, including c-Myc, SP1 and E2, which critically regulate promoter activity. The present study is based on the idea that the hTERT promoter can confer strong tumor-specific transgene expression, minimizing toxicity to normal cells, compared to the universal promoters. The following results were obtained from the present study.\n1) In vitro and in vivo administration of chimeric vectors in which hTERT promoter is linked to apoptosis-inducing genes, such as Caspase, FADD or Bax successfully induce apoptosis in cancer cells with damaging normal cell.\n2) The binary adenovirus system using two vectors, Ad/GT-BAX and Ad/hTERT-GV16 augmented the anti tumor-efficacy.\n3) The transfection of the vector designed to drive MCP-1 gene by hTERT promoter induced the expression of MCP-1 more extensively, compare to CMV-promoter. The multidisciplinary treatment of therapeutic vectors with MCP-1 gene and cisplatin suppressed significantly tumor growth in vivo.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:12470339, 研究期間(年度):2000 – 2002","subitem_description_type":"Other"},{"subitem_description":"出典：「卵巣癌に対するテロメレース活性中心hTERT遺伝子プロモーターを用いた遺伝子治療」研究成果報告書　課題番号12470339\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-12470339/124703392002kenkyu_seika_hokoku_gaiyo/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学系研究科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00063519","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=10127186"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=10127186","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-12470339/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-12470339/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-12470339/124703392002kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-12470339/124703392002kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-21"}],"displaytype":"detail","filename":"ME-PR-INOUE-M-kaken 2004-2p.pdf","filesize":[{"value":"103.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-INOUE-M-kaken 2004-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/57249/files/ME-PR-INOUE-M-kaken 2004-2p.pdf"},"version_id":"0fcc0390-2fa5-4cc0-a544-fcfa5af8b758"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"卵巣癌に対するテロメレース活性中心hTERT遺伝子プロモーターを用いた遺伝子治療","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"卵巣癌に対するテロメレース活性中心hTERT遺伝子プロモーターを用いた遺伝子治療"},{"subitem_title":"Gene therapy against ovarian cancers using hTERT promoter","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2831"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-21"},"publish_date":"2021-10-21","publish_status":"0","recid":"57249","relation_version_is_last":true,"title":["卵巣癌に対するテロメレース活性中心hTERT遺伝子プロモーターを用いた遺伝子治療"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:39:16.734331+00:00"}