{"created":"2023-07-27T06:58:38.312004+00:00","id":57412,"links":{},"metadata":{"_buckets":{"deposit":"bff7a930-3523-4572-960b-7eb0ebbb7ac3"},"_deposit":{"created_by":18,"id":"57412","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"57412"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00057412","sets":["2812:2813:2830"]},"author_link":["584","86269"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-04-18","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2002 – 2003","bibliographic_titles":[{"bibliographic_title":"平成15(2003)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"2003 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"最近、血漿中に微量に存在する核酸をPCR法により増幅して臨床検査に応用しようとする試みが行われている。B細胞性リンパ系悪性腫瘍患者の腫瘍細胞と血漿を用いて、それぞれにおいてIgH-CDR3領域をsemi-nested PCR法で増幅し、その塩基配列の解析を試みた。\nB細胞性リンパ系悪性腫瘍においては,免疫グロブリン遺伝子の同一の再構成を持った細胞が単クローン性に増殖している。この単クローン性増殖を、免疫グロブリンH鎖(IgH)の第3相補性決定部位(CDR3)をPCR法により増幅することで判定でき(IgH-PCR法)、B細胞性リンパ系悪性腫瘍の診断や治療後の微小残存病変のモニタリングに有用とされている。\n血漿を用いたIgH-PCR法のclonalityの検出結果は、B細胞性リンパ系悪性疾患33例中12例(36%)で陽性となり、これまでの報告よりも陽性率が低かった。非特異的バンドが見られたため、低頻度ながら陽性か陰性か判断できない例も見受けられた。非ホジキンリンパ腫では、マントル細胞型リンパ腫で陽性率が高く、濾胞性リンパ腫で低く、病型によって陽性率が異なっていた。さらに、白血病患者およびリンパ腫患者ともに病期が進行している程、陽性率が高かった。一方、健常者やT細胞性リンパ腫、骨髄性白血病では、全例陰性であり、特異性は高かった。\n血漿を用いたIgH-PCR法が陽性であった患者について、血漿と腫瘍細胞のIgH-PCR産物の塩基配列を決定したところ、全症例で両者の塩基配列が一致した。さらに、この塩基配列はこれまでに報告されている疾患に特徴的な塩基配列と相同性が認められた。このことから、血漿中のDNAは明らかに腫瘍細胞から放出されたものであることが証明され,臨床検査としての有用性が示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"In B cell lymphoid malignancies, malignant cells proliferate monoclonally with the same immunoglobulin gene rearrangement.This rearrangement can be detected by the use of the polymerase chain reaction(PCR).Recently, it has been reported that proliferation of clonal cells could be demonstrated by PCR analyzing DNA extracted from patients' plasma with B cell malignancies.The question, however, still remains whether amplified products were derived from tumor cells or not.In this study, using DNA extracted from both malignant cells and plasma of the same patients with B cell leukemia, I attempted to amplify immunoglobulin heavy chain(IgH) complementarity determining region 3(CDR3) by semi-nested PCR and to analyze the sequences respectively.The results showed that sequences of DNA from plasma were identical with that of DNA from tumor cells in all cases.In addition, some of them showed homology to CDR3 that was previously reported.These findings indicate that plasma DNA is released from tumor cells.In the next stage, it is necessary to raise the sensitivity, and if it works, IgH-PCR using plasma DNA might be useful tool for diagnosing patients and evaluating the efficacy of treatment, especially in patients with critical condition under which any biopsies were difficult.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:14572177, 研究期間(年度):2002 – 2003","subitem_description_type":"Other"},{"subitem_description":"出典：「血漿中の腫瘍由来核酸を用いた腫瘍マーカー検査法の確立」研究成果報告書　課題番号14572177\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-14572177/145721772003kenkyu_seika_hokoku_gaiyo/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00063682","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=00160523"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=00160523","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-14572177/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-14572177/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-14572177/145721772003kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-14572177/145721772003kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-22"}],"displaytype":"detail","filename":"ME-PR-OHTAKE-S-kaken 2005-2p.pdf","filesize":[{"value":"106.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-OHTAKE-S-kaken 2005-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/57412/files/ME-PR-OHTAKE-S-kaken 2005-2p.pdf"},"version_id":"6624b36d-946f-4c8f-aa63-4cde4cdae1f2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"血漿中の腫瘍由来核酸を用いた腫瘍マーカー検査法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"血漿中の腫瘍由来核酸を用いた腫瘍マーカー検査法の確立"},{"subitem_title":"Establishment of tumor maker test using tumor-derived nucleic acids in plasma","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2830"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-22"},"publish_date":"2021-10-22","publish_status":"0","recid":"57412","relation_version_is_last":true,"title":["血漿中の腫瘍由来核酸を用いた腫瘍マーカー検査法の確立"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:36:37.366809+00:00"}