{"created":"2023-07-27T06:58:39.032924+00:00","id":57483,"links":{},"metadata":{"_buckets":{"deposit":"86a3115b-0708-4cb2-bbbf-a3c5869ad010"},"_deposit":{"created_by":18,"id":"57483","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"57483"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00057483","sets":["2812:2813:2831"]},"author_link":["20795","95704"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-04-13","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2001 – 2002","bibliographic_titles":[{"bibliographic_title":"平成14(2002)年度 科学研究費補助金 基盤研究(C) 研究報告書概要"},{"bibliographic_title":"2002 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒトや動物の内分泌機能に障害を与える外因性の化学物質、いわゆる内分泌撹乱化学物質が大きな環境問題になっており、大気浮遊粒子状物質(SPM)の内分泌撹乱作用も注目されている。そこで本研究では、金沢市と札幌市において季節毎(4月、7月、10月、1月)にSPMを捕集し、その内分泌撹乱作用(エストロゲン系/アンドロゲン系に及ぼす影響)を培養細胞系を用いて評価するとともに、作用を担う成分について種々検討し、以下の成果を得た。1.何れの試料も抗エストロゲン作用および抗アンドロゲン作用を示した、2.金沢と札幌の何れの都市においても内分泌撹乱作用は、冬期試料が強く、夏期試料が弱いという季節変動を示し、これは冬期には暖房などでエネルギー消費が増大するためと考えられた、3.アリル炭化水素受容体アンタゴニストで共処理するとSPM試料の内分泌撹乱作用が減弱され、SPM試料の内分泌撹乱作用の一部はアリル炭化水素受容体(AhR)のアゴニストとして作用する成分に基づくことが明らかになった、4.環境中に存在する代表的なAhRアゴニストの一つは多環芳香族炭化水素であるので、SPM試料中の多環芳香族炭化水素濃度の分析とそれらの内分泌撹乱作用の評価した結果、SPM試料の内分泌撹乱作用の一部は多環芳香族炭化水素に基づくことが明らかになった、5.競争的ホルモン受容体結合試験および酵母two-hybridアッセイの結果から、SPM中にはエストロゲン受容体/アンドロゲン受容体に結合する成分が存在し、各受容体にアンタゴニストして結合することが明らかになった。","subitem_description_type":"Abstract"},{"subitem_description":"There is an increasing environmental concern on exogenious chemicals that exhibit adverse effects on endocrine systems of human and wild life, so-called endocrine disrupting substances. Endocrine disrupting effects of airborne suspended particulate matter (SPM) has also attracted much attention. Therefore, wecollected SPM in Kanazawa, Ishikawa, and Sapporo, Hokkaido, at April, July, October and January. Then, we assayed their endocrine disrupting effects in cultured human-originated cell lines and investigated the responsible constituents. The results obtained are described below. 1. Everey SPM samples exhibited the antiestrogenic and antiandrogenic effects. 2. At both Kanazawa and Sapporo, SPM samples showed greater effect in winter than in summer. This was considered to be due to increased energy consumption in winter compared with that in summer. 3. The endocrine disrupting effects of SPM samples were redued when the cells were treated with SPM samples in combination with an aryl hydrocarbon receptor (AhR) agonist. This results indicated that the endocrine disrupting effects were due in part to constituents acting as an AhR agonists. 4. Since polycyclic aromatic hydrocarbons (PAHs) are typical AhR agonists in environment, several PAHs were determined for their concentration in SPM samples and assayed for their endocrine disrupting effects. The results revealed that the endocrine disrupting effects of SPM samples are based in part on PAHs. 5. Competitive hormone receptor binding assay and yeast two-hybrid assay revealed that SPM samples contains the constituents capable to bind to estrogen receptor or androgen receptor as antagonists.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:13672342, 研究期間(年度):2001 – 2002","subitem_description_type":"Other"},{"subitem_description":"出典：「大気浮遊粒子状物質の内分泌撹乱作用とその本体」研究成果報告書　課題番号13672342\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-13672342/136723422002kenkyu_seika_hokoku_gaiyo/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学大学院・自然科学研究科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00063753","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=80143915"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=80143915","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-13672342/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-13672342/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-13672342/136723422002kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-13672342/136723422002kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-21"}],"displaytype":"detail","filename":"SC-PR-KIZU-R-kaken 2004-2p.pdf","filesize":[{"value":"100.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-KIZU-R-kaken 2004-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/57483/files/SC-PR-KIZU-R-kaken 2004-2p.pdf"},"version_id":"a533b9c3-8ca4-4a9a-8351-bc60a9a74fd2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"大気浮遊粒子状物質の内分泌撹乱作用とその本体","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"大気浮遊粒子状物質の内分泌撹乱作用とその本体"},{"subitem_title":"Endocrine Disrupting Effects of Airborne Particle Matter and Their Responsible Constituents","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2831"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-21"},"publish_date":"2021-10-21","publish_status":"0","recid":"57483","relation_version_is_last":true,"title":["大気浮遊粒子状物質の内分泌撹乱作用とその本体"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:39:25.456380+00:00"}