{"created":"2023-07-27T06:58:41.194756+00:00","id":57602,"links":{},"metadata":{"_buckets":{"deposit":"2432d6b5-38a4-49e6-a8b8-3ff13993499a"},"_deposit":{"created_by":18,"id":"57602","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"57602"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00057602","sets":["2812:2813:2832"]},"author_link":["49415","24669"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-09-16","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1998 – 2001","bibliographic_titles":[{"bibliographic_title":"平成13(2001)年度 科学研究費補助金 基盤研究(C) 研究概要"},{"bibliographic_title":"2001 Research Project Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究ではヘモグロビン(Hb)の持つアロステリー(協同性)と蛋白質の立体構造変化との関連を知る目的で、主に機能異常を呈する異常血色素を用いてその機能と構造ついて調べた。蛋白質の構造変化は主に紫外円二色性(UVCD)と紫外共鳴ラマン分光法(UVRR)により追求した。研究成果は次の3つにまとめられる。\n1.UVCDによるヘモグロビン四次構造変化に関する研究:Deoxy型のみにみられる287nmの負のCDバンドが何に由来するか、サブユニット接触面に変異を有する異常血色素を用いて調べた(業績1、2)。\n2.異常鎖のヘム鉄が酸化型である異常血色素、HhMのうち、Hb M IwateとHb M Bostonについての研究:いずれのHb Mも異常鎖が酸化されているのみならず、正常鎖の酸素親和性が極端に低い。ヘムの構造および蛋白質の構造について可視および紫外共鳴ラマン分光により追求した(業績3、4)。\n3.UVRRを用いた血色素の構造と機能に関する研究:235nm励起共鳴ラマンスペクトルにおいて、Deoxy→Oxy(or CO)で変化がみられるチロシン(Tyr)およびトリプトファン(Trp)のラマンバンドがどの部位のアミノ酸残基によるか、異常ヘモグロビン、NOおよび金属置換Hbを用いて調べた(業績5〜7)。\n以上の研究結果から、ヘモグロビンの機能発現には、サブユニット接触面にあるチロシンやトリプトファン等の芳香族アミノ酸が、重要な役割を担っていることが判明した。それらは単独ではなく、相乗的に高次構造変化に寄与している事実は大変に興味深い。","subitem_description_type":"Abstract"},{"subitem_description":"In order to address the relationship between oxygen binding function (allostery) and quaternary structure changes of hemoglobin A (Hb A), we have studied on structure and function of abnormal hemoglobin by ultraviolet (UV) circular dichroism (CD) and UV resonance Raman (UVRR) spectroscopy. The results were summarized as follows :\n(1) A study of quaternary structure change of Hb by UVCD : We examined UVCD spectra of abnormal hemoglobin with amino acid substitution at α1-β2 subunit interface (References, 1, 2).\n(1) Heme and protein structure of Hb M Iwate and Hb M Boston characterized by UV and visible resonance Raman study (References, 3, 4).\n(2) Structural and functional relationship of hemoglobin by UVRR : Tyr and Trp RR bands excited with 235nm changed upon deoxy->oxy(or CO) structure transition. We clarified which Tyr or Trp residue contributed to these changes using abnormal hemoglobin, NO and metal-hybrid Hb (References, 5-7).\nFrom these results, we have elucidated that Tyr and/or Trp residues at α1-β2 subunit interface are critically important for hemoglobin allostery. These aromatic residues contribute not independently but cooperatively to higher order protein structure transition of hemoglobin.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:10670115, 研究期間(年度):1998 – 2001","subitem_description_type":"Other"},{"subitem_description":"出典：「変異血色素における機能異常とタンパク質の高次構造変化との関連」研究成果報告書　課題番号10670115\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10670115/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00063872","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=60019578"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=60019578","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10670115/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10670115/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-09-03"}],"displaytype":"detail","filename":"ME-PR-NAGAI-M-kaken 2003-2p.pdf","filesize":[{"value":"99.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-NAGAI-M-kaken 2003-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/57602/files/ME-PR-NAGAI-M-kaken 2003-2p.pdf"},"version_id":"26fa0db9-dfc5-4741-90b1-50592a1a5884"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"変異血色素における機能異常とタンパク質の高次構造変化との関連","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"変異血色素における機能異常とタンパク質の高次構造変化との関連"},{"subitem_title":"Relationship of function and higher order structure of abnormal hemoglobin.","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2832"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-03"},"publish_date":"2021-09-03","publish_status":"0","recid":"57602","relation_version_is_last":true,"title":["変異血色素における機能異常とタンパク質の高次構造変化との関連"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:57:17.323167+00:00"}