{"created":"2023-07-27T06:58:43.175934+00:00","id":57669,"links":{},"metadata":{"_buckets":{"deposit":"80ae9b2f-45aa-45d5-aac0-ef4e552ef5d2"},"_deposit":{"created_by":18,"id":"57669","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"57669"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00057669","sets":["2812:2813:2834"]},"author_link":["15560","95628"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-10-22","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1998 – 1999","bibliographic_titles":[{"bibliographic_title":"平成11(1999)年度 科学研究費補助金 基盤研究(B) 研究成果報告書概要"},{"bibliographic_title":"1999 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ゴルジ体以降の分泌系およびエンドサイトーシス経路における膜蛋白質の選別輸送には一般に、輸送される膜蛋白質の細胞質領域に存在する、特定のアミノ酸配列からなる輸送シグナルを必要とする。輸送シグナルのひとつ、チロシンシグナルは、クラスリン輸送小胞の被覆成分であるAP複合体のμ鎖と結合することにより、膜蛋白質の小胞内への選択的取り込みに寄与する。AP複合体は分子ファミリーを形成しており、また、チロシンシグナルも、エンドサイトーシスをはじめ複数の蛋白質選別輸送に関与する。したがって、それぞれのAP複合体が異なるチロシンシグナルのサブセットと結合することにより、このような輸送の多様性を生み出していると考えられた。そこでyeast2-hybrid法を用い、広範に発現する4種のμ鎖と結合するチロシンシグナルの配列を調べた。その結果、それぞれのμ鎖は異なるチロシンシグナルサブセットと高親和性に結合することが明かとなった。このことから、チロシンシグナルおよびAP複合体の多様性と結合特異性が、膜蛋白質の選別輸送を制御していると考えられる。\nまた我々は、新たなμ鎖ホモログで既知のμ鎖のひとつμ1Aと高い相同性を持つμ1Bを単離した。μ1Bは広く発現するμ1Aと異なり、上皮細胞特異的に発現していた。上皮細胞の細胞膜はtight junctionにより先端面と側基底面に隔てられており、それぞれの細胞膜への膜蛋白質の選別輸送は厳密に制御されている。我々は遺伝子導入の実験から、μ1Bが側基底面細胞膜への膜蛋白質の選別輸送を制御していることを明らかにした。","subitem_description_type":"Abstract"},{"subitem_description":"Selective sorting of membrane proteins in the post-Golgi secretory and endocytic pathways are largely regulated by sorting signals encoded within the cytoplasmic tails of sorted proteins. One of the sorting signals, the tyrosine-based sorting signal, works for selective intake of the sorted molecules into the clathrin-coated vesicles (CCV), by interacting with the μ subunits of AP complexes, a coat component of the CCV. AP complexes consist a protein family, and the tyrosine-based sorting signals are known to be involved in many intracellular sorting pathways including endocytosis and basolateral sorting. This raised the possibility that each μ chain specifically recognizes a subset of the tyrosine-based sorting signals to produce specificity and diversity of the sorting. We utilized a yeast 2-hybrid assay to show this is the case.\nWe cloned a new μ homologue, μ1B, which is highly homologous to one of the ubiquitously expressed μ chains, μ1A. In contrast to μ1A, however, the expression of μ1B is restricted to epithelial cells. Plasma membrane of epithelial cells are physically divided into two domains, apical and basolateral, and membrane proteins are sorted selectively to these two domains. By reconstituting the expression of μ1B into an epithelial cell line lacking the μ1B expression, we were able to show that μ1B is involved in the selective sorting of membrane proteins to the basolateral plasma membrane in epithelial cells.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:10480155, 研究期間(年度):1998 – 1999","subitem_description_type":"Other"},{"subitem_description":"出典：「輸送小胞を介する選択的細胞内蛋白質輸送の制御機構」研究成果報告書　課題番号10480155\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-10480155/104801551999kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00063939","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=50233226"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=50233226","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10480155/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10480155/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-10480155/104801551999kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-10480155/104801551999kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-09-13"}],"displaytype":"detail","filename":"CA-PR-OHNO-H-kaken 2001-2p.pdf","filesize":[{"value":"60.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-OHNO-H-kaken 2001-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/57669/files/CA-PR-OHNO-H-kaken 2001-2p.pdf"},"version_id":"1fcadb6d-eb76-4ef8-86de-b91317896177"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"輸送小胞を介する選択的細胞内蛋白質輸送の制御機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"輸送小胞を介する選択的細胞内蛋白質輸送の制御機構"},{"subitem_title":"Mechanism of regulation of selective intracellular protein sorting by the family of Adaptor (-like) protein complexes","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2834"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-13"},"publish_date":"2021-09-13","publish_status":"0","recid":"57669","relation_version_is_last":true,"title":["輸送小胞を介する選択的細胞内蛋白質輸送の制御機構"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:55:40.869717+00:00"}