@article{oai:kanazawa-u.repo.nii.ac.jp:00058271,
 author = {Kato, Toshiaki and Takeuchi, Lisa and Ono, Yoshihiro and 三木, 理 and Kawara, Osami and 加藤, 敏朗 and 竹内, りさ and 小野, 芳朗 and 三木, 理 and 河原, 長美},
 issue = {7},
 journal = {水環境学会誌, Journal of Japan Society on Water Environment},
 month = {},
 note = {For developing water treatment system to prevent waterborne Cryptosporidiosis, it is necessary to estimate the inactivation of Cryptosporidium. Recently, cell culture model has developed into a tool that can be used for assessing the viability and/or infectivity of Cryptosporidium oocysts. In this study, we investigated to develop quantative and sensitive detection method for determining the infectivity of Cryptosporidium oocysts by combining cell culture with enzyme-linked immunosorbent assay (ELISA). Human ileocecal epitherial cells (HCT-8), which were seeded in gelatin-coated 96-well plates at the density of 1 × 104 cells · well-1 and incubated at 37°C for 48h, were infected with the oocysts. After inoculation, HCT-8 cells were re-incubated with fresh culture media at 37°C for 48h, then were processed for ELISA. The absorbance values in ELISA were increased in an inoculating dose-dependent manner. In the experimental condition, the number of the oocysts from 1 × 102 to 3 × 104 per well was quantitatively detected., 金沢大学理工研究域機械工学系},
 pages = {427--432},
 title = {培養細胞へのCryptosporidium parvumの感染とELISAによる検出},
 volume = {23},
 year = {2000}
}