{"created":"2023-07-27T06:59:04.590357+00:00","id":58271,"links":{},"metadata":{"_buckets":{"deposit":"8ec6e528-8e3c-490b-b498-be0a48fea13e"},"_deposit":{"created_by":18,"id":"58271","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"58271"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00058271","sets":["934:935:936"]},"author_link":["16555","101708","101707","16225","101664","103554","101665","103553","103555","2660"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"432","bibliographicPageStart":"427","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"水環境学会誌"},{"bibliographic_title":"Journal of Japan Society on Water Environment","bibliographic_titleLang":"en"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kato, Toshiaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takeuchi, Lisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Yoshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三木, 理"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kawara, Osami"}],"nameIdentifiers":[{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"For developing water treatment system to prevent waterborne Cryptosporidiosis, it is necessary to estimate the inactivation of Cryptosporidium. Recently, cell culture model has developed into a tool that can be used for assessing the viability and/or infectivity of Cryptosporidium oocysts. In this study, we investigated to develop quantative and sensitive detection method for determining the infectivity of Cryptosporidium oocysts by combining cell culture with enzyme-linked immunosorbent assay (ELISA). Human ileocecal epitherial cells (HCT-8), which were seeded in gelatin-coated 96-well plates at the density of 1 × 104 cells · well-1 and incubated at 37°C for 48h, were infected with the oocysts. After inoculation, HCT-8 cells were re-incubated with fresh culture media at 37°C for 48h, then were processed for ELISA. The absorbance values in ELISA were increased in an inoculating dose-dependent manner. In the experimental condition, the number of the oocysts from 1 × 102 to 3 × 104 per well was quantitatively detected.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域機械工学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00064539","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本水環境学会 Japan Society on Water Environment"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2965/jswe.23.427","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.jstage.jst.go.jp/browse/jswe/-char/ja/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/browse/jswe/-char/ja/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.jswe.or.jp/index-j.html"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jswe.or.jp/index-j.html","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 日本水環境学会 Japan Society on Water Environment"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN10372439","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8958","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1881-3690","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 敏朗"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"竹内, りさ"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小野, 芳朗"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三木, 理"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"河原, 長美"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-24"}],"displaytype":"detail","filename":"TE-PR-MIKI-O-23_427.pdf","filesize":[{"value":"805.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"TE-PR-MIKI-O-23_427.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/58271/files/TE-PR-MIKI-O-23_427.pdf"},"version_id":"a4f4221b-2374-4dcf-afff-1870e8309758"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"培養細胞へのCryptosporidium parvumの感染とELISAによる検出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"培養細胞へのCryptosporidium parvumの感染とELISAによる検出"},{"subitem_title":"Infection of Cryptosporidium parvum Oocysts to Cultured Cells and Detection of the Oocysts by ELISA","subitem_title_language":"en"}]},"item_type_id":"4","owner":"18","path":["936"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-24"},"publish_date":"2022-01-24","publish_status":"0","recid":"58271","relation_version_is_last":true,"title":["培養細胞へのCryptosporidium parvumの感染とELISAによる検出"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:56:13.532759+00:00"}