{"created":"2023-07-27T06:59:24.166736+00:00","id":58971,"links":{},"metadata":{"_buckets":{"deposit":"9793bb20-6f71-4541-ab2e-7b44734e101f"},"_deposit":{"created_by":18,"id":"58971","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"58971"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00058971","sets":["4200:1874:1875"]},"author_link":["78846","22513","87","91215","94248","85011","115","91214","43"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"41","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Molecules"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三代, 憲司"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"柴, 和弘"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"絹谷, 清剛"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"小川, 数馬"}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The purpose of this study is to develop peptide-based platelet-derived growth factor receptor β (PDGFRβ) imaging probes and examine the effects of several linkers, namely un-natural amino acids (D-alanine and β-alanine) and ethylene-glycol (EG), on the properties of Ga-DOTA(linker)-IPLPPPRRPFFK peptides. Seven radiotracers,67 Ga-DOTA-(linker)-IPLPPPRRPFFK peptides, were designed, synthesized, and evaluated. The stability and cell uptake in PDGFRβ positive peptide cells were evaluated in vitro. The biodistribution of [67 Ga]Ga-DOTA-EG2-IPLPPPRRPFFK ([67 Ga]27) and [67 Ga]Ga-DOTA-EG4-IPLPPPRRPFFK ([67 Ga]28), which were selected based on in vitro stability in murine plasma and cell uptake rates, were determined in BxPC3-luc-bearing nu/nu mice. Seven67 Ga-labeled peptides were successfully synthesized with high radiochemical yields (>85%) and purities (>99%). All evaluated radiotracers were stable in PBS (pH 7.4) at 37◦ C. However, only [67 Ga]27 and [67 Ga]28 remained more than 75% after incubation in murine plasma at 37◦ C for 1 h. [67 Ga]27 exhibited the highest BxPC3-luc cell uptake among the prepared radiolabeled peptides. As regards the results of the biodistribution experiments, the tumor-to-blood ratios of [67 Ga]27 and [67 Ga]28 at 1 h post-injection were 2.61 ± 0.75 and 2.05 ± 0.77, respectively. Co-injection of [67 Ga]27 and an excess amount of IPLPPPRRPFFK peptide as a blocking agent can significantly decrease this ratio. However, tumor accumulation was not considered sufficient. Therefore, further probe modification is required to assess tumor accumulation for in vivo imaging. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This.","subitem_description_type":"Abstract"}]},"item_4_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"CC-BY 4.0","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学疾患モデル総合研究センター","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00065233","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI AG"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/molecules26010041","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.mdpi.com/journal/molecules"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.mdpi.com/journal/molecules","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/)."}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1420-3049","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Effendi, Nurmaya"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Mishiro, Kenji"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Shiba, Kazuhiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kinuya, Seigo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ogawa, Kazuma"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-27"}],"displaytype":"detail","filename":"ME-PR-SHIBA-K-26.41.pdf","filesize":[{"value":"897.7 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SHIBA-K-26.41.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/58971/files/ME-PR-SHIBA-K-26.41.pdf"},"version_id":"035ad400-871b-4fdb-af34-22fb073f838b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of radiogallium-labeled peptides for platelet-derived growth factor receptor β (Pdgfrβ) imaging: Influence of different linkers","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of radiogallium-labeled peptides for platelet-derived growth factor receptor β (Pdgfrβ) imaging: Influence of different linkers"}]},"item_type_id":"4","owner":"18","path":["1875"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-27"},"publish_date":"2022-01-27","publish_status":"0","recid":"58971","relation_version_is_last":true,"title":["Development of radiogallium-labeled peptides for platelet-derived growth factor receptor β (Pdgfrβ) imaging: Influence of different linkers"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:17:48.091456+00:00"}