{"created":"2023-07-27T06:59:24.394081+00:00","id":58977,"links":{},"metadata":{"_buckets":{"deposit":"60206883-9fb2-4215-ab32-4c5315dcb9c9"},"_deposit":{"created_by":18,"id":"58977","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"58977"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00058977","sets":["4200:1874:1875"]},"author_link":["103814","85162","80506","78846","22513","1880","87","98107","80169","103816","85011","80505","80168","115","43"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-01-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"e0146719","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小阪, 孝史"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"北村, 暘二"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"小川, 数馬"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"瀧, 淳一"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"絹谷, 清剛"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"柴, 和弘"}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose\nTo develop a superior VAChT imaging probe for SPECT, radiolabeled (-)-OIDV and (+)-OIDV were isolated and investigated for differences in their binding affinity and selectivity to VAChT, as well as their in vivo activities.\n\nProcedures\nRadioiodinated o-iodo-trans-decalinvesamicol ([125I]OIDV) has a high binding affinity for vesicular acetylcholine transporter (VAChT) both in vitro and in vivo. Racemic [125I]OIDV was separated into its two optical isomers (-)-[125I]OIDV and (+)-[125I]OIDV by HPLC. To investigate VAChT binding affinity (Ki) of two OIDV isomers, in vitro binding assays were performed. In vivo biodistribution study of each [125I]OIDV isomer in blood, brain regions and major organs of rats was performed at 2,30 and 60 min post-injection. In vivo blocking study were performed to reveal the binding selectivity of two [125I]OIDV isomers to VAChT in vivo. Ex vivo autoradiography were performed to reveal the regional brain distribution of two [125I]OIDV isomers and (-)-[123I]OIDV for SPECT at 60 min postinjection.\n\nResults\nVAChT binding affinity (Ki) of (-)-[125I]OIDV and (+)-[125I]OIDV was 22.1 nM and 79.0 nM, respectively. At 2 min post-injection, accumulation of (-)-[125I]OIDV was the same as that of (+)-[125I]OIDV. However, (+)-[125I]OIDV clearance from the brain was faster than (-)-[125I]OIDV. At 30 min post-injection, accumulation of (-)-[125I]OIDV (0.62 ± 0.10%ID/g) was higher than (+)-[125I]OIDV (0.46 ± 0.07%ID/g) in the cortex. Inhibition of OIDV binding showed that (-)-[125I]OIDV was selectively accumulated in regions known to express VAChT in the rat brain, and ex vivo autoradiography further confirmed these results showing similar accumulation of (-)-[125I]OIDV in these regions. Furthermore, (-)-[123I]OIDV for SPECT showed the same regional brain distribution as (-)-[125I]OIDV.\n\nConclusion\nThese results suggest that radioiodinated (-)-OIDV may be a potentially useful tool for studying presynaptic cholinergic neurons in the brain.","subitem_description_type":"Abstract"}]},"item_4_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"CC-BY 4.0","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学疾患モデル総合研究センター","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00065239","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0146719","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.plosone.org/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.plosone.org/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146719"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146719","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2016 Uno et al.This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited., Published by the Public Library of Science"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uno, Izumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kozaka, Takashi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Miwa, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitamura, Yoji"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Azim, Mohammad Anwar-ul"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogawa, Kazuma"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Taki, Junichi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kinuya, Seigo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Shiba, Kazuhiro"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-28"}],"displaytype":"detail","filename":"ME-PR-SHIBA-K-0146719.pdf","filesize":[{"value":"297.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SHIBA-K-0146719.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/58977/files/ME-PR-SHIBA-K-0146719.pdf"},"version_id":"12f03c64-b22e-4163-8079-de1550fe32c9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In vivo differences between two optical isomers of radioiodinated o-iodo-transdecalinvesamicol for Use as a radioligand for the vesicular acetylcholine transporter","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vivo differences between two optical isomers of radioiodinated o-iodo-transdecalinvesamicol for Use as a radioligand for the vesicular acetylcholine transporter"}]},"item_type_id":"4","owner":"18","path":["1875"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-28"},"publish_date":"2022-01-28","publish_status":"0","recid":"58977","relation_version_is_last":true,"title":["In vivo differences between two optical isomers of radioiodinated o-iodo-transdecalinvesamicol for Use as a radioligand for the vesicular acetylcholine transporter"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:18:08.367350+00:00"}