{"created":"2023-07-27T06:59:24.485261+00:00","id":58979,"links":{},"metadata":{"_buckets":{"deposit":"ce113bcc-282e-4271-b8c1-87a778296328"},"_deposit":{"created_by":18,"id":"58979","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"58979"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00058979","sets":["4200:1874:1875"]},"author_link":["27222","103836","20906","78846","78849","87","103829","103830","80169","85011","1851","103832","27393","80168","27391","102","43"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-12-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageStart":"e81191","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_4_creator_36":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小川, 数馬"}],"nameIdentifiers":[{"nameIdentifier":"87","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30347471","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30347471"},{"nameIdentifier":"30347471","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=30347471"},{"nameIdentifier":"30347471","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030347471"}]},{"creatorNames":[{"creatorName":"北村, 暘二"}],"nameIdentifiers":[{"nameIdentifier":"80168","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10368483","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10368483"}]},{"creatorNames":[{"creatorName":"小野口, 昌久"}],"nameIdentifiers":[{"nameIdentifier":"1851","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30283120","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30283120"}]},{"creatorNames":[{"creatorName":"柴, 和弘"}],"nameIdentifiers":[{"nameIdentifier":"43","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40143929","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40143929"},{"nameIdentifier":"40143929","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=40143929"},{"nameIdentifier":"40143929","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000040143929"}]},{"creatorNames":[{"creatorName":"小谷, 明"}],"nameIdentifiers":[{"nameIdentifier":"102","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60143913","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60143913"},{"nameIdentifier":"60143913","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=60143913"},{"nameIdentifier":"60143913","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000060143913"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"99mTc-HYNIC-annexin A5 can be considered as a benchmark in the field of apoptosis imaging. However, 99mTc-HYNIC-annexin A5 has characteristics of high uptake and long retention in non-target tissues such as kidney and liver. To minimize this problem, we developed a novel 99mTc-labeled annexin A5 using a bis(hydroxamamide) derivative [C3(BHam)2] as a bifunctional chelating agent, and evaluated its usefulness as an imaging agent for detecting apoptosis. The amino group of C3(BHam)2 was converted to a maleimide group, and was coupled to thiol groups of annexin A5 pretreated with 2-iminothiolane. 99mTc labeling was performed by a ligand exchange reaction with 99mTc-glucoheptonate. Biodistribution experiments for both 99mTc-C3(BHam)2-annexin A5 and 99mTc-HYNIC-annexin A5 were performed in normal mice. In addition, in tumor-bearing mice, the relationship between the therapeutic effects of chemotherapy (5-FU) and the tumor accumulation of 99mTc-C 3(BHam)2-annexin A5 just after the first treatment of 5-FU was evaluated. 99mTc-C3(BHam)2-annexin A5 was prepared with a radiochemical purity of over 95%. In biodistribution experiments, 99mTc-C3(BHam)2-annexin A5 had a much lower kidney accumulation of radioactivity than 99mTc-HYNIC- annexin A5. In the organs for metabolism, such as liver and kidney, radioactivity after the injection of 99mTc-HYNIC-annexin A5 was residual for a long time. On the other hand, radioactivity after the injection of 99mTc-C3(BHam)2-annexin A5 gradually decreased. In therapeutic experiments, tumor growth in the mice treated with 5-FU was significantly inhibited. Accumulation of 99mTc-C 3(BHam)2-annexin A5 in tumors significantly increased after 5-FU treatment. The accumulation of radioactivity in tumor correlated positively with the counts of TUNEL-positive cells. These findings suggest that 99mTc-C3(BHam)2-annexin A5 may contribute to the efficient detection of apoptotic tumor response after chemotherapy. © 2013 Ogawa et al.","subitem_description_type":"Abstract"}]},"item_4_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"CC-BY 4.0","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学疾患モデル総合研究センター","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00065241","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0081191","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.plosone.org/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.plosone.org/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081191"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081191","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2013 Ogawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ogawa, Kazuma"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ohtsuki, Katsuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Tomomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aoki, Miho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama, Morio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitamura, Yoji"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ono, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueda, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Doue, Tomoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onoguchi, Masahisa"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Shiba, Kazuhiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Odani, Akira"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-31"}],"displaytype":"detail","filename":"ME-PR-SHIBA-K-e81191.pdf","filesize":[{"value":"444.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SHIBA-K-e81191.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/58979/files/ME-PR-SHIBA-K-e81191.pdf"},"version_id":"7478fd8d-7084-473a-8f1d-7bb9c032186e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development and evaluation of a novel 99mTc-labeled annexin A5 for early detection of response to chemotherapy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development and evaluation of a novel 99mTc-labeled annexin A5 for early detection of response to chemotherapy"}]},"item_type_id":"4","owner":"18","path":["1875"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-31"},"publish_date":"2022-01-31","publish_status":"0","recid":"58979","relation_version_is_last":true,"title":["Development and evaluation of a novel 99mTc-labeled annexin A5 for early detection of response to chemotherapy"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:18:01.072806+00:00"}