{"created":"2023-07-27T06:59:40.240759+00:00","id":59329,"links":{},"metadata":{"_buckets":{"deposit":"d7cf882a-09dc-4a6d-ac8e-dc875778659a"},"_deposit":{"created_by":18,"id":"59329","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"59329"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00059329","sets":["2812:2813:4085"]},"author_link":["97924"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-05-31","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"3p.","bibliographicVolumeNumber":"2021-04-01 – 2022-03-31","bibliographic_titles":[{"bibliographic_title":"令和3(2021)年度 科学研究費補助金 奨励研究 研究成果報告書"},{"bibliographic_title":"2021 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hamabe, Toshihide"}],"nameIdentifiers":[{"nameIdentifier":"97924","nameIdentifierScheme":"WEKO"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"in vitro実験系において、胃癌細胞株MKN45にTGF-β1を添加し、悪性化を誘導した後、細胞からmRNAを抽出、培養上清からExosomeを回収した。その結果、 Exosome-Free FBSを含む培地を使用した場合にもMKN45の悪性化誘導は可能であり、10mLの細胞培養上清からmiRNA発現解析に十分なExosomeが回収可能であることを確認した。現在、悪性化誘導をした胃癌細胞で発現量が増加しているmiRNAに対してmiRNA-mRNA統合解析を行なっており、胃癌細胞由来exo-miRNAが標的としているmRNAの絞り込みを進めている。","subitem_description_type":"Abstract"},{"subitem_description":"体液中に存在するmiRNAをがんのバイオマーカーとして利用することで、早期がんを含むがんの疑いのある人をスクリーニングする一次検診が可能になることが期待されている。本研究により、胃癌の新規バイオマーカーの候補miRNAがエクソソームを媒介して体液中に分泌される可能性を検討することができる。現在は、胃癌の新規バイオマーカーの候補miRNAが標的とするmRNAの絞り込みをするめている。本解析により、胃癌の進行を抑制するmRNAを標的とする候補miRNAを見出すことができれば、より低侵襲で簡便な診断精度の高い検査法の開発に貢献できる可能性がある。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:21H04178, 研究期間:2021-04-01 – 2022-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「進行性胃癌由来エクソソームmiRNA(exo-miRNA)の探索と診断への応用」課題番号21H04178（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-21H04178/21H04178seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学総合技術部(医)","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00065591","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=21H04178"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=21H04178","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-21H04178/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-21H04178/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-21H04178/21H04178seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-21H04178/21H04178seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"浜辺, 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