@article{oai:kanazawa-u.repo.nii.ac.jp:00000595,
 author = {Masuda, Kazumi and Takakura, Hisashi and Furuichi, Yasuro and Iwase, Satoshi and Jue, Thomas},
 journal = {Advances in experimental medicine and biology},
 month = {Jan},
 note = {In order to obtain evidence that Mb releases O(2) during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (Delta[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of Delta[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the Delta[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO(2) as workload increases., 金沢大学人間社会研究域人間科学系},
 pages = {323--328},
 title = {NIRS measurement of O(2) dynamics in contracting blood and buffer perfused hindlimb muscle.},
 volume = {662},
 year = {2010}
}