{"created":"2023-07-27T07:00:01.277287+00:00","id":59806,"links":{},"metadata":{"_buckets":{"deposit":"9bf63bf2-3f52-438c-b951-1bb6eee7b35a"},"_deposit":{"created_by":18,"id":"59806","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"59806"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00059806","sets":["2812:2813:2836"]},"author_link":["48001","31022"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"3p.","bibliographicVolumeNumber":"1996 – 1997","bibliographic_titles":[{"bibliographic_title":"平成9(1997)年度 科学研究費補助金 国際学術研究 研究成果報告書概要"},{"bibliographic_title":"1997 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okada, Yasunori"}],"nameIdentifiers":[{"nameIdentifier":"48001","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00115221","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000000115221"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マトリックスメタロプロテアーゼ(MMP)遺伝子ファミリーのうちでも、MMP-2(ゼラチナーゼA)はがん細胞浸潤・転移や関節破壊と密接に関係しており、生体内での作用機序が注目されている。MMP-2の活性化酵素として細胞膜貫通型MMP(MT-MMP)がクローニングされ、MT-MMP/MMP-2の相互作用による細胞外マトリックス分解機序解析が最重要研究課題となっている。本研究における主な研究成果は、以下の如くである。\n1.ヒト乳癌組織における潜在型MMP-2の活性化は、MTI-MMP発現と正の相関を示した。一方、MT2-MMPとMT3-MMPの発現は弱く、潜在型MMP-2活性化にMTI-MMPが中心的役割を果たすことが明らかとなった。甲状腺癌組織においても、潜在型MMP-2活性化率はリンパ節転移と正の相関を示し、MTI-MMPがその活性化主役を演じていた。\n2.ヒト変形性関節症と慢性関節リウマチの関節軟骨では潜在型MMP-2の活性化がみられ、MTI-MMPの発現と正の相関を示した。また、MTI-MMP発現陽性軟骨細胞の細胞膜成分は、潜在型MMP-2を活性化した。さらに、MTI-MMPの軟骨細胞による発現は軟骨の破壊程度とよく相関した。\n3.リコンビナントMTI-MMPの生化学的性質を調べたところ、本酵素は潜在型MMP-2を活性化するだけでなく、I,II,III型コラーゲン、ゼラチン、プロテオグリカン、フィブロネクチン、ヴィトロネクチン、ラミニンを分解した。MT3-MMPについても検討したところ、I型コラーゲンを除いてほぼ同様な活性を示した。\n4.MTI-MMP遺伝子を軟骨組織で特異的に発現する遺伝子導入マウスを作製しており、現在その表現型を調べている。今後、本マウスをMMP-2遺伝子欠損マウスと交配し、表現型を解析する予定である。","subitem_description_type":"Abstract"},{"subitem_description":"Among the matrix metalloproteinase (MMP) gene family members, MMP-2 (gelatinase A) is believed to be involved in cancer invasion and metastasis and joint destruction, and thus its function in vivo is important. Membrane-type MMPs (MT-MMPs) were recently cloned as acribators of proMMP-2 and the degradation mechanism of extracellular matrix by the MT-MMPs/MMP-2 system is one of the key projects in the field of MMP research. In the present studies, we have demonstrated that proMMP-2 activation is mediated by MT1-MMP in the human invasive breast carcinomas and human thyroid carcinomas. In the human osteoarthritic and rheumatoid arhtritic cartilages, MT1-MMP also playd a major role in the activation of proMMP-2, showing a positive correlation with cartilage destruction. We also revealed that MT1-MMP is an extracellular matrix-degrading proteinase capable of digesting interstitial collagens and aggrecan as well as an activator of proMMP-2. MT3-MMP had a similar acitivity against these substrates except for type I collagen. Transgenic mice expressing MT1-MMP specifically in the cartilages are being made and analyzes of their phenotypes are now under way. These mice will be back crossed with MMP-2 knockout mice which had been made by a Japanese group and their phenotypes will be examined.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:08044262, 研究期間(年度):1996 – 1997","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「遺伝子導入・欠損マウスによる細胞外マトリックス代謝の解析」課題番号08044262\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08044262/080442621997kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"慶應義塾大学 / 金沢大学がん研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00066057","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00115221"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00115221","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-08044262/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-08044262/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08044262/080442621997kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08044262/080442621997kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岡田, 保典"}],"nameIdentifiers":[{"nameIdentifier":"31022","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00115221","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00115221"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-26"}],"displaytype":"detail","filename":"CA-PR-OKADA-Y-kaken 1999-3p.pdf","filesize":[{"value":"122.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-OKADA-Y-kaken 1999-3p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/59806/files/CA-PR-OKADA-Y-kaken 1999-3p.pdf"},"version_id":"8e2a3ddd-8849-459c-80cd-ff4ab69491bf"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"遺伝子導入・欠損マウスによる細胞外マトリックス代謝の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"遺伝子導入・欠損マウスによる細胞外マトリックス代謝の解析"},{"subitem_title":"Analyzes on ECM metabolism in transgenic and knockout mice","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2836"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-26"},"publish_date":"2022-05-26","publish_status":"0","recid":"59806","relation_version_is_last":true,"title":["遺伝子導入・欠損マウスによる細胞外マトリックス代謝の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T06:37:22.633344+00:00"}