{"created":"2023-07-27T07:00:06.038732+00:00","id":59909,"links":{},"metadata":{"_buckets":{"deposit":"9de43d18-9d1a-4846-9184-0031100ded8f"},"_deposit":{"created_by":18,"id":"59909","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"59909"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00059909","sets":["2812:2813:2836"]},"author_link":["100603","21832"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1996 – 1997","bibliographic_titles":[{"bibliographic_title":"平成9(1997)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"1997 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Fas/APO-1(CD95)はFas ligand(FasL)やFas抗体でアポトーシスを引き起こすことで知られる細胞膜受容体の一つである。以前、神経膠腫の壊死巣周囲細胞にFasが主に発現し、そのFasがアポトーシスを引き起こし、壊死巣を形成するのではないかと報告した。また、細胞死の経路構成し、抗癌剤やFas抗体、放射線で誘発されるアポトーシスにおいて誘導されるカスパーゼファミリーであるICE{interleukin-1β-converting enzyme(ICE)}やICH-1(ICE/CED-3 homologue-1)、CPP32は本研究においては極めて重要な因子である。本研究においては、神経膠腫13例に対してFasL、ICE、ICH-1およびCPP32の発現をRT-PCR、Western blot法、さらに免疫組織化学的手法を用いて、それらのmRNAと蛋白を観察した。RT-PCR法では神経膠腫全例にFasL、ICH-1、CPP32の発現がみられた。免疫染色ではFasLは神経膠芽腫全例の腫瘍細胞膜に過剰発現がみられた。このことは、もし神経膠腫がFasを発現すれば、アポトーシスが誘導できることを示唆する。ICE、ICH-1、およびCPP32の蛋白レベルの過剰発現は、神経膠腫の悪性化と共に認められた。さらに、ICH-1とCPP32の神経膠芽腫における過剰発現は、神経膠芽腫の病理学的特徴である壊死巣の形成に深く関与し、重要な機能を担うことが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Fas/APO-1 (CD95) is a cell surface receptor that mediates apoptosis when it reacts with Fas ligand (FasL) or Fas antibody. I previously reported that Fas expression in predominantly induced in perinecrotic glioma cells, suggesting that Fas induction is associated with apoptosis and necrosis formation, a histological hallmark of glioblastomas. Cysteine proteases of caspase family {interleukin-1 beta-converting enzyme (ICE)} have been implicated as components of cell death pathway and have been reported to involved in Fas, chemotherapeutic agents, and radiation-induced apoptosis. In this study, I assessed the expression lf FasL,ICE,ICE/CED-3 homologue-IL (ICH-1), and CPP32/Yama/apopain in 13 cases of primary astrocytic brain tumors (two low grade astrocytomas, five anaplastic astrocytomas, and six glioblastomas) by reverse transcription (RT) -PCR,Western blot analysis, and immunohistochemistry. RT-PCR revealed that all astrocytic brain tumors express FasL.Immunohistochemically, FasL was predominantly expressed on the plasma membrane of glioma cells. These results suggest that FasL expression is common in human astrocytic brain tumors and may cause apoptosis of glioma cells if Fas expression is induced. The frequency of ICE,ICH-1, and CPP32 overexpression appears to correlate with the malignacy grade of astrocytic brain tumors. Furthermore, ICH-1 and CPP32 overexpression may play an important role in the pathogenesis of necrosis, which is one of the histological hallmarks of glioblastoma.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:08671571, 研究期間(年度):1996 – 1997","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「悪性神経膠腫に対するFas/Apo-1(CD95)標的治療の基礎的研究」課題番号08671571\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08671571/086715711997kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部附属病院","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00066160","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40211362"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40211362","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-08671571/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-08671571/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08671571/086715711997kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08671571/086715711997kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-30"}],"displaytype":"detail","filename":"HO-PR-TACHIBANA-O-kaken 1999-2p.pdf","filesize":[{"value":"78.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-TACHIBANA-O-kaken 1999-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/59909/files/HO-PR-TACHIBANA-O-kaken 1999-2p.pdf"},"version_id":"a421946a-f332-455c-b2ab-7a7c5f78a808"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"悪性神経膠腫に対するFas/Apo-1(CD95)標的治療の基礎的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"悪性神経膠腫に対するFas/Apo-1(CD95)標的治療の基礎的研究"},{"subitem_title":"The role of Fas/Fas ligand system in human","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2836"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-30"},"publish_date":"2022-05-30","publish_status":"0","recid":"59909","relation_version_is_last":true,"title":["悪性神経膠腫に対するFas/Apo-1(CD95)標的治療の基礎的研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:59:21.982507+00:00"}