{"created":"2023-07-27T07:00:07.046977+00:00","id":59931,"links":{},"metadata":{"_buckets":{"deposit":"4dfb2714-b2aa-4e01-8b69-6b2dcced2410"},"_deposit":{"created_by":18,"id":"59931","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"59931"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00059931","sets":["2812:2813:2836"]},"author_link":["105611"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1996 – 1997","bibliographic_titles":[{"bibliographic_title":"平成9(1997)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"1997 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"化学的シナプス伝達はシナプス小胞がシナプス前終末形質膜膜への結合、プライミング、融合そしてexocytosisからなる。この課程の制御機構を明らかにするため関与する遺伝子を同定し、分子性状を明らかにしつつある。本研究ではunc-64遺伝子について調べ次の結果を得た。\n1。nuc-64遺伝子が変異すると、シナプス伝達が阻害され、運動性異常となる。\n2。nuc-64遺伝子はsyntaxin homologをcodeする。この蛋白はC末に膜貫通領域があり、のこりの大部分はシナプス前終末に存在する。N末に3helical領域があり、他の蛋白と強い相互作用をすると推定された。\n3。C.elegans syntaxinはUNC-18及びUNC-13と高親和性に結合する。その祭結合には、syntaxin全領域を必要とした。\n以上からUNC-64がコードするC.elegans syntaxinはシナプス伝達では中心的役割をしていることが明かになった。","subitem_description_type":"Abstract"},{"subitem_description":"The unc-18 gene contributes to the synaptic transmission. n-Sec-1, the mammalian UNC-18 homolog is believed to be associated with a synaptic plasma membrane protein prior to synaptic vesicle docling. If this were the case for the C.elegans UNC-18, the gene mutations would result in constitutive fusions of synaptic vesicles. However, the gene mutations result in the accunulation of synaptic vesicles and neurotransmitters. To examine the conflicting observations, we analyzed the binding of wild-type and mutant UNC-18 to syntaxin.\nUNC-18 is a hydrophilic, globular, and neutral protein with pI 6.95. In the Sf21 cells the protein is predominantly localized in the cytoplasm, indicating its intrinsic solubility. To investigate further the inracellular distribution of UNC-18, we isolated synaptic vesicles by the percoll gradient fractionation. UNC-18 was detected on synaptic vesicles by Western blotting. We cloned C.elegans cDNAs encoding the mammalian syntaxinA homolog (Ce syntaxin). We tested the binding of UNC-18 to GST-Ce syntaxin fusion protein. UNC-18 binds to the recombinant syntaxin in vitro with high affinity. These findings indicate that the protein is periplasmic associating with both synaptic and plasma membrane, although intrinsically soluble. In vesicle traffic UNC-18 may be regulator with plasma membrane through syntaxin. UNC-18 may cause a conformational change of syntaxin. Therefore the protein allows it to bind to synaptic vesicles.\nTo test hypotesis, we sequenced cDNAs of 15 unc-18 mutants. We found two missense mutations and the binding ability of mutant UNC-18 proteins to Ce syntaxin is being teted.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:08680838, 研究期間(年度):1996 – 1997","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「神経系シャペロン様因子によるシナプス伝達制御機構」課題番号08680838\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08680838/086808381997kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00066182","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40019617"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40019617","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-08680838/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-08680838/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08680838/086808381997kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-08680838/086808381997kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-30"}],"displaytype":"detail","filename":"ME-PR-HOSONO-R-kaken 1999-2p.pdf","filesize":[{"value":"98.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-HOSONO-R-kaken 1999-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/59931/files/ME-PR-HOSONO-R-kaken 1999-2p.pdf"},"version_id":"ffe101ae-a024-490b-b496-a49c0348ae88"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"神経系シャペロン様因子によるシナプス伝達制御機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"神経系シャペロン様因子によるシナプス伝達制御機構"},{"subitem_title":"A regulatory mechanism of synaptic transmission by a chaperone-like factor","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2836"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-30"},"publish_date":"2022-05-30","publish_status":"0","recid":"59931","relation_version_is_last":true,"title":["神経系シャペロン様因子によるシナプス伝達制御機構"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:59:45.721844+00:00"}