{"created":"2023-07-27T07:00:08.881182+00:00","id":59971,"links":{},"metadata":{"_buckets":{"deposit":"d28a51ea-5b33-40fc-87b3-ef59abac91b0"},"_deposit":{"created_by":18,"id":"59971","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"59971"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00059971","sets":["2812:2813:2837"]},"author_link":["48001","31022"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-08","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"3p.","bibliographicVolumeNumber":"1995 – 1996","bibliographic_titles":[{"bibliographic_title":"平成8(1996)年度 科学研究費補助金 基盤研究(B) 研究成果報告書概要"},{"bibliographic_title":"1996 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okada, Yasunori"}],"nameIdentifiers":[{"nameIdentifier":"48001","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00115221","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000000115221"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マトリックスメタロプロテアーゼ(Matrix metalloproteinase=MMP)遺伝子ファミリーは、一次構造が明らかにされた15種類の酵素から構成されており、共通のインヒビターであるtissue inhibitors of metalloproteinases (TIMP)にも4種類(TIMP-1, -2, -3, -4)が区別されている。本研究では、膜型MMP (MT1-MMP)が変形性関節症の関節軟骨に強く発現し、潜在型MMP-2活性化に関与することを示した。また、これまで潜在型MMP-2活性化酵素としての側面しか知られていなかったMT1-MMPが、細胞外マトリックス分解酵素活性を有することを初めて実証した。さらに、MMP-7とMMP-8に対するサンドイッチイムノアッセイ系を新たに開発した。一方、病的関節軟骨においてはcartilage matrix proteinやSPARCなどの新規細胞外マトリックスの過剰発現が生じることを実証し、MMPによるマトリックス分解に続発した生体反応の一部と理解される。実際、軟骨プロテオグリカンの一種であるデコリンのMMPによる分解の結果、細胞外マトリックス産生促進因子であるTGF-βの遊離が生じることを示した。現在、MT1-MMP活性のTIMP-2による阻害機構について検討するとともに、関節軟骨におけるTIMP-1, 2, 3, 4の発現のパターンと軟骨破壊との関連について解析している。","subitem_description_type":"Abstract"},{"subitem_description":"Matrix metalloproteinases (MMPs) are composed of at least 15 different molecules and their activities are strictly regulated by their common tissue inhibitors of metalloproteinases (TIMP-1,2,3,4). In the present studies, we have demonstrated that membrane-type 1 matrix metalloproteinase (MT1-MMP) is highly expressed in the human osteoarthritic cartilage showing a positive correlation with activation of proMMP-2 (progelatinase A). We have also revealed that MT1-MMP is an extracellular matrix (ECM) -degrading proteinase capable of digesting interstitial collagens and aggrecan as well as an activator of proMMP-2. In osteoarthritis and rheumatoid arthritis, cartilage matrix protein and SPARC were overexpressed, suggesting that it may be a tissue reaction secondary to the degradation of the ECM components by MMPs expressed in the cartilages. Actually, TGF-beta which stimulates ECM production in various mesenchymal cells was released after degradation of the TGF-beta/decorin complex by MMPs. To determine the production levels, sandwich enzyme immunoassays for MMP-7 and MMP-8 were also developed. Research projects on the mechanisms of MT1-MMP inhibition by TIMP-2 and expression of TIMP-1,2,3,4 in osteoarthritic and rheumatoid arthritic cartilages are now under way.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:07457049, 研究期間(年度):1995 – 1996","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「マトリックスメタロプロテアーゼによる関節破壊に関する研究」課題番号07457049\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07457049/074570491996kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00066222","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00115221"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00115221","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457049/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457049/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07457049/074570491996kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07457049/074570491996kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岡田, 保典"}],"nameIdentifiers":[{"nameIdentifier":"31022","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00115221","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00115221"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-09"}],"displaytype":"detail","filename":"CA-PR-OKADA-Y-kaken 1999-3p.pdf","filesize":[{"value":"105.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-OKADA-Y-kaken 1999-3p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/59971/files/CA-PR-OKADA-Y-kaken 1999-3p.pdf"},"version_id":"065fdf54-fd82-40ab-8207-0ea40834324b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"マトリックスメタロプロテアーゼによる関節破壊に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マトリックスメタロプロテアーゼによる関節破壊に関する研究"},{"subitem_title":"Studies on the destruction of articular cartilage by matrix metalloproteinases","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2837"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-06-09"},"publish_date":"2022-06-09","publish_status":"0","recid":"59971","relation_version_is_last":true,"title":["マトリックスメタロプロテアーゼによる関節破壊に関する研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T06:38:20.362330+00:00"}