{"created":"2023-07-27T07:00:12.350066+00:00","id":60048,"links":{},"metadata":{"_buckets":{"deposit":"37b385ff-dfd0-4c20-89a0-b09682c46f0e"},"_deposit":{"created_by":18,"id":"60048","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60048"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060048","sets":["2812:2813:2836"]},"author_link":["30366","105723"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1995 – 1997","bibliographic_titles":[{"bibliographic_title":"平成9(1997)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"1997 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"分裂病と健常人でNMDAR1遺伝子内の多型について関連研究を行うための前段階として、データベースにある既知のNMDAR1遺伝子内の多型が、日本人の健常人でもみられるか、PCR-RFLP法を用いて検討した。対象は、32名の健常人で男性18名、女性14名である。データベースに報告されているNMDAR1遺伝子内の多型のうち、PCR-RFLP法で検出可能な2つの部位でPCRを行った。さらに、それぞれを制限酵素処理してバンドのパターンを比較した。PCRの結果、1つの領域では予期したものより長いバンドが、全例でみられた。また、もう1つの領域では予期したものより短いバンドが、全例でみられた。いずれの領域も、制限酵素処理後のパターンに差はみられなかった。PCR産物の長さの違いは、既知のNMDAR1遺伝子の塩基配列の人種差によると考えられた。多型については、日本人では、欧米人でみられる多型がないか、あってもその頻度が低いと考えられ、精神分裂病との関連研究を行う上で適さないと考えられた。","subitem_description_type":"Abstract"},{"subitem_description":"As a preliminary study, it was investigated in normal Japanese whether there was a mutation in the gene encoding the human N-methyl-D-aspartate receptor (NMDAR1) or not. Subjects were 32 normal volunteers of Japanese, who consisted of 18 males and 14 females. It has been alr eady reported that there are two portions showing a mutation in the gene encoding the human NMDAR1 in normal Eur opeans and Americans (12694 in exon 21 and 6254 in exon 7). In the present study, the existence of the already-known mutation in the two portions was separately examined with PCR-RFLP methods in the nor mal Japanese. As a result, no mutation was detected in either of the two portions. However, in one of the two portions (12694 in exon 21), the length of DNA band finally produced with restriction endonuclease treatment following PCR was long beyond our expectations from the already-known report. Futher, in another portion (6254 in exon 7), the length of DNA band after restriction endonuclease treatment was short against our expectations.\nIt was consider ed that the mutation in the gene encoding NMDAR1 was absent in Japanese or existed only in a much low frequency. There fore, it seemed that the analysis of mutation in the gene encoding NMDAR1 was not appropriate in schizophr enia research. Further, the difference in the length of DNA band as the PCR-RFLP-induced products was presumed to be due to the racial difference between Japanese and Weaterners.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:07671057, 研究期間(年度):1995 – 1997","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「精神分裂病におけるNMDA型受容体サブユニットNMDAR1遺伝子の解析」課題番号07671057\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07671057/076710571997kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00066299","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=60110532"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=60110532","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671057/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671057/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07671057/076710571997kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07671057/076710571997kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-03"}],"displaytype":"detail","filename":"ME-PR-JIBIKI-I-kaken 1999-2p.pdf","filesize":[{"value":"74.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-JIBIKI-I-kaken 1999-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/60048/files/ME-PR-JIBIKI-I-kaken 1999-2p.pdf"},"version_id":"7b002830-3fc2-41c7-9f5d-5033b7768be2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"精神分裂病におけるNMDA型受容体サブユニットNMDAR1遺伝子の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"精神分裂病におけるNMDA型受容体サブユニットNMDAR1遺伝子の解析"},{"subitem_title":"Analysis of gene encoding the human n-methyl-d-aspartate receptor (nmdar1) in schizophrenics","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2836"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-06-03"},"publish_date":"2022-06-03","publish_status":"0","recid":"60048","relation_version_is_last":true,"title":["精神分裂病におけるNMDA型受容体サブユニットNMDAR1遺伝子の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:57:21.022263+00:00"}