{"created":"2023-07-27T07:00:16.767447+00:00","id":60145,"links":{},"metadata":{"_buckets":{"deposit":"65bc911a-56bf-4977-bb5f-abd6f2e9776e"},"_deposit":{"created_by":18,"id":"60145","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60145"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060145","sets":["2812:2813:2838"]},"author_link":["74","97510"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-03-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"3p.","bibliographicVolumeNumber":"1994 – 1995","bibliographic_titles":[{"bibliographic_title":"平成7(1995)年度 科学研究費補助金 一般研究(B) 研究成果報告書概要"},{"bibliographic_title":"1995 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"インターロイキン-2(IL-2)、IL-2リセプターα鎖、IL-6、組織適合抗原クラスI及びII、免疫グロブリンκ鎖、等の免疫制御に関わる複数の遺伝子の発現調節にNF-κBは重要な役割を果している。さらに、NF-κBファミリーやそのインヒビターのノックアウトマウスの研究などから、NF-κB、c-Rel、RelB、等のNF-κBファミリーのメンバーが様々な免疫系の細胞の分化・増殖・アポトーシスに重要な役割を果たしていると考えられているが、その機序は明らかではない。特に、c-rel癌遺伝子は、主にリンパ系の細胞で発現しており、c-relのウイルス癌遺伝子v-relがトリのリンパ系腫瘍の発症に深く関与している事から、c-relがリンパ系細胞の分化・増殖に重要な役割を果していると考えられているが、現在のところその免疫系における役割は明らかではない。これらの点を明らかにすることを目的として、NF-κBファミリーの活性化の分子機構について研究した。その結果、NF-κBファミリーの活性化には、そのインヒビターのプロテアゾームによる蛋白分解が必須のステップであるが、我々はNF-κBファミリーの重要なインヒビターの一つの_P105 (NF-κB_P50サブユニットの前駆体)の蛋白分解がサイクリン依存性キナーゼによる_P105のC末部の燐酸化に依存して起こることを明らかにした。さらに_P105のC末部と相互作用する細胞内因子として、細胞微小管をかいして細胞内で様々な小胞体や物質の輸送や細胞分裂に関係するキネシンスーパーファミリーの一員と思われる蛋白がクローニングされた。この新しいキネシン様蛋白は約1400個のアミノ酸からなり、N末部にキネシンスーパーファミリーに特徴的なATP結合や微少管との結合に関与するモチーフが認められ、胸腺細胞などの未熟なTリンパ細胞に特に強く発現していた。今後、この新しいキネシン様蛋白のNF-κBファミリーの活性化における役割や、T細胞の分化・成熟や活性化における役割について明らかにしていく。","subitem_description_type":"Abstract"},{"subitem_description":"NF-kappaB is aninducible transcription factor which plays an essential role in the activation of various immune genes, such as genes for interleukin-2 (IL-2) IL-2 receptor alpha chain, IL-6 and MHC molecules. The results of recent studies indicate that NF-kappaB and its family members play an important role in the activation, growth, differentiation and apoptosis of various lymphoid cells. To study roles of NF-kappaB family members in the immune system, we studied how the activation of NF-kappaB family members is regulated and what cellular factors are involved. An unique feature of NF-kappaB is that it pre-exists in the cytoplasm in an inactive form complexed with inhibitory proteins. One of these inhibitors is p105 which is a precursor for one of subunits (p50) of the NF-kappaB transcription factor and the proteolytic processing of its inhibitory C-terminal region is required for generation of active NF-kappaB.We found that the p105 C-terminal region is phosphorylated in vivo on Ser-894 and Ser-908, which are potential phosphorylation sites in vitro for proline-directed serine/threonine kinase such as cyclin-dependent kinase. Furthermore, the mutation of these in vivo phosphorylation sites retards p105 processing/degradation in vivo, suggesting that p105 processing/degradation is regulated in phosphorylation-dependent manners. To further identify cellular factors involved in p105 phosphorylation and processing, we carried out Far-Western and two-hybrid cloning using the p105 C-terminal region as a bait. One of several cDNA cloned found to encode a new member of the kinesin superfamily : the N-terminal portion of the predicted amino acid sequence contained ATP and microtubules binding motifs which are hallmarks of motor proteins and are well conserved in the kinesin superfamily. Interestingly, the expression of the gene encoding this new kinesin superfamily member which interacts with the C-terminal region of p105 in vitro was confined to thymus and testis. We are currently studing what roles this new kinesin superfamily member plays in the regulation of NF-kappaB activation.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:06454217, 研究期間(年度):1994 – 1995","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「NF-KBの制御に関わるT細胞リセプターのクローニングとその免疫制御における役割」課題番号06454217\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-06454217/064542171995kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00066396","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=60115285"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=60115285","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-06454217/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-06454217/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-06454217/064542171995kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-06454217/064542171995kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山本, 健一"}],"nameIdentifiers":[{"nameIdentifier":"74","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60115285","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60115285"},{"nameIdentifier":"60115285","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000060115285"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-17"}],"displaytype":"detail","filename":"CA-PR-YAMAMOTO-K-kaken 1997-3p.pdf","filesize":[{"value":"99.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-YAMAMOTO-K-kaken 1997-3p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/60145/files/CA-PR-YAMAMOTO-K-kaken 1997-3p.pdf"},"version_id":"22bc8216-33d6-4a74-90ba-b7f67b970013"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"NF-KBの制御に関わるT細胞リセプターのクローニングとその免疫制御における役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"NF-KBの制御に関わるT細胞リセプターのクローニングとその免疫制御における役割"},{"subitem_title":"Molecular cloning of a new kinesin superfamily member interacting with a NF-kappaB inhibitor","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2838"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-06-17"},"publish_date":"2022-06-17","publish_status":"0","recid":"60145","relation_version_is_last":true,"title":["NF-KBの制御に関わるT細胞リセプターのクローニングとその免疫制御における役割"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T06:40:00.418481+00:00"}