{"created":"2023-07-27T07:00:26.034225+00:00","id":60365,"links":{},"metadata":{"_buckets":{"deposit":"96f4ca93-c8cd-423e-a469-e1c14a518ac0"},"_deposit":{"created_by":18,"id":"60365","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60365"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060365","sets":["2812:2813:2840"]},"author_link":["21071","106076"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1993","bibliographic_titles":[{"bibliographic_title":"平成5(1993)年度 科学研究費補助金 奨励研究(A) 研究概要"},{"bibliographic_title":"1993 Research Project Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1)ヒト胆嚢粘膜移植片(microexplant)の作製:胆石症の診断で,開腹手術により摘出された胆嚢を,直ちに無菌的に切開し,胆汁を流出させた.粘膜面に付着した胆汁成分を滅菌ガ-ゼで拭き取り,氷冷ハンクス液で充分洗浄した後,肉眼的に著変のない部位を選んで,粟粒大の粘膜片を線維筋層を含まないように剥離切除し,微小移植片(microexplant)を得た.\n2)ヒト胆嚢粘膜上皮の初代培養系の確立:作製したmicroexplantをI型コラーゲン・ゲル上に静置し,15%牛胎児血清添加培養液(DMEM/HamF12)を重層し,5%CO_2インキュベータ-内に初代培養した.ほとんどのmicroexplantはコラーゲン・ゲルに接着し,培養2日にはヒト胆嚢上皮細胞が周囲のコラーゲン・ゲル上に増殖するのが位相差顕微鏡で確認できた.上皮細胞は培養7日までコラーゲン・ゲル表面を進展増殖し,培養面積が増加したが,以後はコラーゲン・ゲルから剥離し,培養面積は縮小した.移植片が小さいほど間質細胞の増殖は少なく,1〜2週間では上皮の増殖に与える影響は少なかった.上皮細胞の形態は扁平であり,in vivoのごとく円柱状のものはみられなかった.また,粘液産生像は認められなかった.\n3)培養上皮細胞へのHLA-DRおよび接着分子の誘導:培養4日目に培養液中にインターフェロン-gamma(100ng/ml)を添加して一週間培養し,培養上皮細胞にHLA-DRおよびICAM-Iの発現誘導を試みたが,凍結切片を使用した酵素抗体法では検出できなかった.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:05770144, 研究期間(年度):1993","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「ヒト胆道上皮細胞のコラーゲン・ゲル上継代培養による純粋培養法の確立」課題番号05770144\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-05770144/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00066613","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=10205635"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=10205635","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-05770144/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-05770144/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-04"}],"displaytype":"detail","filename":"ME-PR-SAITO-K-kaken 2016-2p.pdf","filesize":[{"value":"135.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SAITO-K-kaken 2016-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/60365/files/ME-PR-SAITO-K-kaken 2016-2p.pdf"},"version_id":"84499776-b5c2-44c4-8c2a-d16d5ae3091d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ヒト胆道上皮細胞のコラーゲン・ゲル上継代培養による純粋培養法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト胆道上皮細胞のコラーゲン・ゲル上継代培養による純粋培養法の確立"}]},"item_type_id":"9","owner":"18","path":["2840"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-07-04"},"publish_date":"2022-07-04","publish_status":"0","recid":"60365","relation_version_is_last":true,"title":["ヒト胆道上皮細胞のコラーゲン・ゲル上継代培養による純粋培養法の確立"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:41:00.780360+00:00"}